Team:BIT

From 2013.igem.org

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<p><img src="https://static.igem.org/mediawiki/2013/7/7a/BITflag.gif" width="1024" height="150" border="0" usemap="#Map">
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     <td width="752"><p class="Cap1"><span class="Cap"><span class="Cap">A Brief Introduction of Integrated Sensor for Detection of Milk Product</span></span></p></td>
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     <td width="753"><p class="Cap1">A Brief Introduction of Integrated Sensor</p>
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      <p class="Cap1"> for Detection of Milk Product</p></td>
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     <td><span class="Cap1">A<span class="con">A<span class="Con"><span class="con1"><span class="con1"><span class="Con">s</span></span></span></span></span></span> <span class="con"><span class="con1"><span class="Con"><span class="con1"><span class="Con"><span class="con1"><span class="Cap">we know, antibiotics are widely used in modern industry to prevent the infection of cow breasts. However, the residual of antibiotics and other components, such as Cr (VI) which is widely used in the recycle of proteins from leather, will endanger the health of customers. Therefore, it is necessary to find solution to identify these harmful chemicals, but the method of timely detection of harmful chemicals in products still remains unsolved. <br />
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     <td><span class="Cap2"><span class="Con"><span class="Bot"><span class="cn"><span class="asd"><span class="Cap2"><span class="Cap2"><span class="Cap2">&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span></span></span></span></span></span><span class="Cap">As</span> <span class="Con"><span class="Bot"><span class="cn"><span class="asd"><span class="Cap2"><span class="Cap2"><span class="Cap2"><span class="con1">we know, antibiotics are widely used in modern industry to prevent the infection of cow breasts. However, the residual of antibiotics and other components, such as Cr (VI) which is widely used in the recycle of proteins from leather, will endanger the health of customers. Therefore, it is necessary to find solution to identify these harmful chemicals, but the method of timely detection of harmful chemicals in products still remains unsolved. <br />
&nbsp;&nbsp;&nbsp;&nbsp;To solve this problem, our sensor has been divided into three, respectively, of <a href="https://2013.igem.org/Team:BIT/Project#1">hexavalent chromium, tetracycline and β-lactam</a> there is a response, for each sensor, we are all assembled in a downstream of the <a href="https://2013.igem.org/Team:BIT/Project#2">amplification block</a> (amplifier) ​​and the <a href="https://2013.igem.org/Team:BIT/Project#3">control block </a>(controller), the output signal to be amplified, by adjusting the concentration of IPTG to achieve the adjustment of magnification. Amplifier by the T7 RNA polymerase gene and T7 promoter composition control block is through the downstream of the T7 promoter plus lacO and the addition of regular expression lacI, by adjusting the concentration of IPTG, lacO then adjust the boot situation achieved, the final response module for the green fluorescent protein.<br />
&nbsp;&nbsp;&nbsp;&nbsp;To solve this problem, our sensor has been divided into three, respectively, of <a href="https://2013.igem.org/Team:BIT/Project#1">hexavalent chromium, tetracycline and β-lactam</a> there is a response, for each sensor, we are all assembled in a downstream of the <a href="https://2013.igem.org/Team:BIT/Project#2">amplification block</a> (amplifier) ​​and the <a href="https://2013.igem.org/Team:BIT/Project#3">control block </a>(controller), the output signal to be amplified, by adjusting the concentration of IPTG to achieve the adjustment of magnification. Amplifier by the T7 RNA polymerase gene and T7 promoter composition control block is through the downstream of the T7 promoter plus lacO and the addition of regular expression lacI, by adjusting the concentration of IPTG, lacO then adjust the boot situation achieved, the final response module for the green fluorescent protein.<br />
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&nbsp;&nbsp;&nbsp;&nbsp;In order to achieve real-time detection, we also made a <a href="https://2013.igem.org/Team:BIT/Project#4">biochip</a> which provided a reaction room for both engineering bacteria and samples. Now we are able to detect all these three harmful chemicals with a<a href="https://2013.igem.org/Team:BIT/Project#5"> hand-held electronic equipment</a> made by ourselves. </span></span></span></span></span></span></span><span class="con1"><span class="Con"><span class="con1"><span class="Con"><span class="con1"><span class="Cap"></span></span></span></span></span></span></td>
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&nbsp;&nbsp;&nbsp;&nbsp;In order to achieve real-time detection, we also made a <a href="https://2013.igem.org/Team:BIT/Project#4">biochip</a> which provided a reaction room for both engineering bacteria and samples. Now we are able to detect all these three harmful chemicals with a<a href="https://2013.igem.org/Team:BIT/Project#5"> hand-held electronic equipment</a> made by ourselves. </span></span></span></span></span></span></span></span></td>
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  <span class="Bot"><span>Beijing Institutte of Technology &copy; 2013 </span>| <a href="http://www.bit.edu.cn">Privacy Policy</a></p>
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raduation. As we all know, biology is developing all the time. He  hopes he can know biology more deep by this time iGEM.<br />
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    <td width="471">Zhou Rui is a student who has been majoring in Biotechnology since 2011. When she was a freshman, she joined the lab, mastered the basic experimental technology and laid a solid foundation for the future research. From experiments, she harvests a lot of knowledge which is hard to learn from books and improves the basic experimental skills and creative thinking, fully realizes the rigorous, serious and pragmatic spirit of scientific research. Participating in this project, she will continue this kind of realistic attitude and perform each experiment more seriously.<br />
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    <td width="471">Xingjian Zhang,  Biomedical Engineering junior undergraduates, head of microfluidic chip group,  is also the contact person of BIT-iGEM team, whose motto is &quot;Life  is a continuous improvement journey&quot;. He loves music and  sports. He is a band drummer and also a football team striker. He takes part in  this competition in order to improve his personal ability by learning from  other&rsquo;s advantage and to gain more experience after enjoying the time of this  competition. He expects for this communication and looks forward to meet with  every kind of friends in different culture background.<br />
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    <td width="471">Yunjie Qiu is a junior  majoring in Biotechnology at BIT. She is interested in stem cells research and  microorganism circuit. Besides iGEM, she is doing a related research at college  lab about induced pluripotent stem cells. She would like to learn more about  synthetic biology and do a project from the ground with her team in the process  of competition. In her space, Qiu enjoys playing badminton and watching movies.<br />
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    <td width="471">Mengran Tao is an  undergraduate student majoring in Biotechnology. He participates in the group  of the Chromium ion biosensor and biological chip. People drink milk every day,  so he wishes their biological monitor could help people build the safety of the  milk. He has made lots of friends during the competition. The feeling of  working together to achieve one goal is wonderful. He enjoys it and he is also  interested in the synthetic biology. iGEM gives him a chance to cultivate the  team spirit and enhance his professional knowledge. His hobby is playing  basketball and playing the guitar, so the NBA and music accompany him in the  free time. Anyone who likes NBA or music can make friends with him.<br />
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    <td width="471"><p>Zhenyu Li is an undergraduate student who  has been majoring in Electronic Packaging since 2011. Although he is not a  professional biology student, he still has deep love for the mystery of it. He  is good at electronics and mathematics. He is responsible for the hardware part  of this project with another one. He has great passion and confidence.<br />
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    <td width="471"> <p>Deyu Zhang, who is the member responsible  for the biological hardware ,Video , and the core of our web construction, is an  undergraduate in grade two coming from BIT.<br /><br />
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    <td width="471">Chenghao Liu is an undergraduate  who has been majoring in Bioengineering since 2010. He is responsible for the  human resource of our team. As a guy who enjoys video games, he also likes to  read science fictions. In order to improve his abilities of English and management,  he joined us.<br />
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    <td width="471"><p>Siyu Feng is a senior student  majoring in Biotechnology, who joined in this team as the webmaster. Besides  academic achievements, she also excels in art, both hand drawing and computer  graphics. She believes these training and skills could benefit her in her  future career. After graduation, she hopes to continue graduate studies in  Molecular Biology and finally receive PhD degree as &ldquo;People who have Dreams.<br />
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    <td width="471"><p>Jing Gao is an undergraduate who has been  majoring in Bioengineering since 2010. She is responsible for the publicity of  our team. She is interested in movies and travel. In order to improve her  abilities of design and English as well as broaden her vision, she joined us. </p>
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    <td width="471"> <p>Xiaoyu Zhou is an undergraduate student  studying Bioengineering. She is responsible for managing financial affairs in  BIT IGEM team. She has many kinds of hobbies such as shopping, sleeping and so  on. She joined this team because she can learn a lot from this competition.</p>
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    <td width="471"><p>Wei Qin is an undergraduate student  studying Bioengineering. He is the  non-experimental manager of BIT IGEM team. He manages this  team in every aspect. He has many kinds of hobbies such as mountaineering,  bicycling and so on. He joined this team because he can learn a lot from this  competition.</p>
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    <td width="471"><p>Kefu Liu, one of the  four advisers in our team, is in his second year as a Master candidate in the  discipline of neurobiology and molecular biology, he offers guide for our works  in the cultivation of Bacterial and molecule biology&rsquo;s experiments.<br />
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    <td width="471"><p>Runhong Lei, one of  the four advisers in our team, is in his third year as PhD candidate in the  discipline of cytology, he offers guide for our experiments on cells.<br />
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    <td width="471"><p>Yan Man, one of the  four advisers in our team, is in his third year as a PhD candidate in the  discipline of biochip, she offers guide for our chip manufacturing works.<br />
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    <td width="471"><p>Qin Kuiwei, one of  the four advisers in our team, is in his third year as a PhD candidate in the  discipline of biochip. He offer guide for our chip manufacturing works.<br />
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    <td width="471"><p>Yulin Deng, one of  the three instructors of our team, is the dean of the School of Life Sciences  at Bit.<br />
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    <td width="471"><p>Hong Qing, one of the  three instructors of our team, is a professor at BIT.<br />
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    <td width="471"><p>Hong Ma, one of the three instructors of our team, is a professor at BIT.<br />
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    <td width="471"><p>Yifei Wang, the secretary of Youth League committee of the school of life science in Bit, takes charge of the over-all consideration and proper arrangement for our team.<br />
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Revision as of 14:21, 8 September 2013

iGEM BIT

 

A Brief Introduction of Integrated Sensor

for Detection of Milk Product
     As we know, antibiotics are widely used in modern industry to prevent the infection of cow breasts. However, the residual of antibiotics and other components, such as Cr (VI) which is widely used in the recycle of proteins from leather, will endanger the health of customers. Therefore, it is necessary to find solution to identify these harmful chemicals, but the method of timely detection of harmful chemicals in products still remains unsolved. 
    To solve this problem, our sensor has been divided into three, respectively, of hexavalent chromium, tetracycline and β-lactam there is a response, for each sensor, we are all assembled in a downstream of the amplification block (amplifier) ​​and the control block (controller), the output signal to be amplified, by adjusting the concentration of IPTG to achieve the adjustment of magnification. Amplifier by the T7 RNA polymerase gene and T7 promoter composition control block is through the downstream of the T7 promoter plus lacO and the addition of regular expression lacI, by adjusting the concentration of IPTG, lacO then adjust the boot situation achieved, the final response module for the green fluorescent protein.
    In order to achieve real-time detection, we also made a biochip which provided a reaction room for both engineering bacteria and samples. Now we are able to detect all these three harmful chemicals with a hand-held electronic equipment made by ourselves.
























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