Team:BIT/project biosensors
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Revision as of 07:53, 13 September 2013
A Detailed Introduction of Integrated Sensor for Detection of Milk Product |
Beta-lactam detection device Background Beta-lactam biosensor is aimed to create a biosensor that can be applied in practical life. It is useful for citizens to know what they drink and what they buy for their little babies are qualified and hygienic. While there are traditional methods to detect beta-lactam antibiotics, such as enzyme-linked immunosorbent assay (ELISA) and ECLIPSE50, all these methods have to rely on laboratories which are equipped with precise instruments. In order to solve the problem, our Beta-lactam biosensor is designed to be used on on-site detection in a few hours by users without special training. Device The bla operon has been found that is induced by beta-lactam. This device will work to detect the concentration of Beta-Lactam in dairy products. At the same time, we designed another two devices to decrease the detection limit. The chr promoter was strongly induced by chromate or dichromate, but it was completely unresponsive to Cr(III), oxidants, sulfate, or other oxyanions. Plasmid reporter experiments identified ChrB as a chromate-sensing regulator of chr expression. According to this evidence, we designed three kinds of devices working in E.coli (DH5α) to build Cr(VI)-biosensor. The chr promoter has a weak constitutive expression without chromate, while it is strongly induced to express. Device 2
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