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Team:Berkeley/Applications
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<div class = "heading-large"><a name="Characterization of Indigo Biosynthesis">Characterization of Indigo Biosynthesis</a></div> | <div class = "heading-large"><a name="Characterization of Indigo Biosynthesis">Characterization of Indigo Biosynthesis</a></div> | ||
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<div class = "heading"><a name="Indigo Titer">Background</a></div> | <div class = "heading"><a name="Indigo Titer">Background</a></div> | ||
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<div class = "heading"><a name="Indigo Titer">Our Novel System</a></div> | <div class = "heading"><a name="Indigo Titer">Our Novel System</a></div> | ||
<p>We will be utilizing our biological dyeing process to design our reporter system. In our reporter system, we will have our glucosidase enzyme that can be “turned off” or “on”, a build up store of indican in the cell, and receptors to detect the signal. Thus, when the signal is detected, our glucosidase enzyme will be “turned on,” and will subsequently convert the already present indican into indoxyl. Indoxyl will rapidly dimerize in the presence in the oxygen, allowing us to visually detect indigo as a result. This process is faster than the conventional mechanisms that require transcriptional initiation on the reporter upon the detection of some substrate for the following reasons: </p> | <p>We will be utilizing our biological dyeing process to design our reporter system. In our reporter system, we will have our glucosidase enzyme that can be “turned off” or “on”, a build up store of indican in the cell, and receptors to detect the signal. Thus, when the signal is detected, our glucosidase enzyme will be “turned on,” and will subsequently convert the already present indican into indoxyl. Indoxyl will rapidly dimerize in the presence in the oxygen, allowing us to visually detect indigo as a result. This process is faster than the conventional mechanisms that require transcriptional initiation on the reporter upon the detection of some substrate for the following reasons: </p> | ||
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Revision as of 00:08, 18 October 2013
Earlier in the summer, we looked into potential applications utilizing with our biological indigo dyeing process. One application we envisioned was a fast and novel biosensor mechanism with our biological process. To do this, we will first build up a store of indican within cell, and when the sensor is activated with a signal, our glucosidase enzyme will turn the indican back into indigo.
Current biosensors require transcriptional initiation on the reporter upon the detection of some substrate. While effective, this process can take many hours (in most cases 12+ hours) to see the reporter! In iGEM, several teams have used the biosensor approach in their iGEM projects developing biosensors ranging from cyanide and heavy metal sensors to rotting meat volatile sensors. These teams used a variety of colorimetric reporter elements as well to signify the detection of their substrate of interest ranging from fluorescent proteins to a variety of pigments. All of these projects however dependent on transcription initiation on the reporter after the signal is detected. We have devised a novel mechanism to see our reporter compound, indigo, in a matter of minutes!
We will be utilizing our biological dyeing process to design our reporter system. In our reporter system, we will have our glucosidase enzyme that can be “turned off” or “on”, a build up store of indican in the cell, and receptors to detect the signal. Thus, when the signal is detected, our glucosidase enzyme will be “turned on,” and will subsequently convert the already present indican into indoxyl. Indoxyl will rapidly dimerize in the presence in the oxygen, allowing us to visually detect indigo as a result. This process is faster than the conventional mechanisms that require transcriptional initiation on the reporter upon the detection of some substrate for the following reasons:
