Team:Berkeley/Safety

From 2013.igem.org

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<li> Risk Group: 1 <br>
<li> Risk Group: 1 <br>
<li> Risk Group Source Link: http://www.absa.org/riskgroups/bacteriasearch.php?genus=Escherichia&species=coli <br>
<li> Risk Group Source Link: http://www.absa.org/riskgroups/bacteriasearch.php?genus=Escherichia&species=coli <br>
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<li> Disease risk to humans? If so, which disease?: "E. coli K-12 is not considered a human or animal pathogen nor is it toxicogenic. Any concerns for E. coli K-12 in terms of health considerations are mitigated by its poor ability to colonize the colon and establish infections."  (http://epa.gov/oppt/biotech/pubs/fra/fra004.htm). <br>
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<li> Disease risk to humans? If so, which disease?: "E. coli K-12 is not considered a human or animal pathogen nor is it toxicogenic. Any concerns for E. coli K-12 in terms of health considerations are mitigated by its poor ability to colonize the colon and establish infections (http://epa.gov/oppt/biotech/pubs/fra/fra004.htm). <br>
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<li> Species: S. cerevisiae <br>
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<li> Strain: S288C <br>
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<li> Risk Group: 1 <br>
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<li> Risk Group Source Link: http://www.accessdata.fda.gov/scripts/fcn/gras_notices/grn000422.pdf <br>
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<li> Disease risk to humans? If so, which disease?: S.cerevisiae is a GRAS organism (Generally recognized as Safe). "Saccharomyces, as a genus, present low risk to human health or the environment. Criteria used to differentiate between species are based on their ability to utilize specific carbohydrates without relevance to pathogenicity." (http://www.epa.gov/biotech_rule/pubs/fra/fra002.htm). <br>
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Revision as of 03:12, 14 September 2013

Safety
1) Please describe the chassis organism(s) you will be using for this project. If you will be using more than one chassis organism, provide information on each of them:

  • Species: E. coli
  • Strain: TG1 and Epi300
  • Risk Group: 1
  • Risk Group Source Link: http://www.absa.org/riskgroups/bacteriasearch.php?genus=Escherichia&species=coli
  • Disease risk to humans? If so, which disease?: "E. coli K-12 is not considered a human or animal pathogen nor is it toxicogenic. Any concerns for E. coli K-12 in terms of health considerations are mitigated by its poor ability to colonize the colon and establish infections (http://epa.gov/oppt/biotech/pubs/fra/fra004.htm).
  • Species: S. cerevisiae
  • Strain: S288C
  • Risk Group: 1
  • Risk Group Source Link: http://www.accessdata.fda.gov/scripts/fcn/gras_notices/grn000422.pdf
  • Disease risk to humans? If so, which disease?: S.cerevisiae is a GRAS organism (Generally recognized as Safe). "Saccharomyces, as a genus, present low risk to human health or the environment. Criteria used to differentiate between species are based on their ability to utilize specific carbohydrates without relevance to pathogenicity." (http://www.epa.gov/biotech_rule/pubs/fra/fra002.htm).



2) Highest Risk Group Listed:



3) List and describe all new or modified coding regions you will be using in your project. (If you use parts from the 2013 iGEM Distribution without modifying them, you do not need to list those parts.)



4) Do the biological materials used in your lab work pose any of the following risks? Please describe.

5) . If your project moved from a small-scale lab study to become widely used as a commercial/industrial product, what new risks might arise? (Consider the different categories of risks that are listed in parts a-d of the previous question.) Also, what risks might arise if the knowledge you generate or the methods you develop became widely available?



6) Does your project include any design features to address safety risks?



7) What safety training have you received (or plan to receive in the future)? Provide a brief description, and a link to your institution’s safety training requirements, if available.



8) Under what biosafety provisions will/do you work?

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