Team:Buenos Aires

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<center style="font-family:'Vollkorn', serif; color: white;"> Wetlab work at UBA labs </center></div>
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<center style="font-family:'Vollkorn', serif; color: white;">We chose for the chassis the cyanobacteria Synechocystis PCC 6803, because it is a photoautotroph and is regulated by circadian rhythms.</center></div>
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<center style="font-family:'Vollkorn', serif; color: white;">Using the lux operon and circadian promoters, we designed two plasmids: the Luciferase generator is to produce bioluminiscence, and the substrate generator recovers substainably the substrates necessary for the reactions.</center></div>
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<center style="font-family:'Vollkorn', serif; color: white;">We achieved to transform Synechocystis with the luciferase generator plasmid, and verificated light emission in a luminometer.</center></div>
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<center style="font-family:'Vollkorn', serif; color: white;">Al being lit by bioluminescent bacteria! </center></div>
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Revision as of 21:31, 27 September 2013

Project: Luxilla - Pontificia Universidad Católica de Chile, iGEM 2012

iGem Buenos Aires

Wetlab work at UBA labs
We chose for the chassis the cyanobacteria Synechocystis PCC 6803, because it is a photoautotroph and is regulated by circadian rhythms.
Using the lux operon and circadian promoters, we designed two plasmids: the Luciferase generator is to produce bioluminiscence, and the substrate generator recovers substainably the substrates necessary for the reactions.
We achieved to transform Synechocystis with the luciferase generator plasmid, and verificated light emission in a luminometer.
Al being lit by bioluminescent bacteria!
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Buenos Aires

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