Team:Ciencias-UNAM

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Project description
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<div id="u47_rtf"><p style="text-align:left;"><span style="font-family:Museo Slab;font-size:20px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;">THE PROJECT</span></p></div>
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<div id="u49_rtf"><p style="text-align:left;"><span style="font-family:Museo Slab;font-size:20px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;">THE PEOPLE</span></p></div>
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<div id="u51_rtf"><p style="text-align:left;"><span style="font-family:Museo Slab;font-size:20px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;">PARTS</span></p></div>
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<div id="u53_rtf"><p style="text-align:left;"><span style="font-family:Museo Slab;font-size:20px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;">ACHIEVEMENTS</span></p></div>
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<div id="u54_rtf"><p align="justify"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;">This year's project is an <i>In vivo</i> production of an antimicrobial peptide regulated by <i>quorum sensing</i>. We designed a synthetic system in <i>E. coli</i> to express an antimicrobial peptide (Cathelicidin LL-37) <i>in vivo</i> only in the presence of a quorum sensing molecule (AI-2) and expel it with an efflux pump in order to act against targeted gastrointestinal pathogens.</span></p></div>
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<div id="u55_rtf"><p align="justify"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;">We are the team from Facultad de Ciencias, UNAM in Mexico City. Our team brings together students and teachers from all over the country, with various backgrounds and interests.</span></p></div>
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<div id="u56_rtf"><p align="justify"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;">We submitted several devices mainly to test and use the following parts:<br><b>Cathelicidin LL-37:</b> An antimicrobial peptide against bacterial infection.<br> <b>MarA:</b> A peptide responsible for an efflux pump in <i>E. coli</i> that is related to its resistance to cathelicidin and similar peptides.</span></p></div>
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<div id="u57_rtf"><p align="justify"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;">The project we developed this year yielded several results. We achieved to characterise  and contribute to the iGEM Registry. In this section we present what we think we are our best and main results.</span></p></div>
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<div id="u58_rtf"><p align="justify"><span style="font-family:Arial;font-size:13px;font-weight:bold;font-style:normal;text-decoration:none;color:#666666;">Read the abstract</span></p></div>
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<div id="u59_rtf"><p align="justify;"><span style="font-family:Arial;font-size:13px;font-weight:bold;font-style:normal;text-decoration:none;color:#666666;">Meet the team</span></p></div>
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<div id="u60_rtf"><p align="justify"><span style="font-family:Arial;font-size:13px;font-weight:bold;font-style:normal;text-decoration:none;color:#666666;">Read more...</span></p></div>
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<div id="u61_rtf"><p align="justify"><span style="font-family:Arial;font-size:13px;font-weight:bold;font-style:normal;text-decoration:none;color:#666666;">Read more...</span></p></div>
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The human peptide LL-37 is a cationic antimicrobial peptide with activity against Gram-negative and Gram-positive bacteria. It has been shown to protect against ''Helicobacter pylori'' infection and its related gastritis, besides of inhibiting the growth of other pathogenic bacteria in the gastrointestinal tract such as ''Salmonella typhimurium'' and ''Escherichia coli''.  
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Most of the current synthetic expression systems for LL-37 depend on the construction of soluble fusion partners to avoid cytotoxic effects of the antimicrobial peptide in the ''E. coli'' host strain. However, these fusion systems require additional cleavage steps using enzymatic or chemical methods, which makes them impossible to express an active LL-37 peptide in vivo. In order to create a resistant host that can export LL-37 to the media, we intend to overexpress the ''E. coli'' acrAB and tolC operons, which activate the AcrAB-TolC efflux pump, a mechanism that has been related with resistance to this and similar antimicrobial peptides by expulsion.  
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Our aim is to create a system in which ''E. coli'' expels LL-37 only when'' H. pylori o''r other pathogenic bacteria, such as'' S. typhimurium'', ''E. coli'' or'' V. cholerae'', are present. In order to do this we are using the LsrA promoter, which allows transcription in the presence of AI-2, a molecule produced by bacteria to communicate via quorum-sensing. ''E. coli'' also produces AI-2, so we designed an antisense RNA with specific secondary structure to inhibit the translation of LuxS, the enzyme responsible of the production of AI-2 in '''E. coli.''
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|[[Image:Ciencias-UNAM_team.png|right|frame|Your team picture]]
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!align="center"|[[Team:Ciencias-UNAM/Team|Team]]
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Latest revision as of 04:20, 28 September 2013

Home

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PROJECT

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THE PROJECT

THE PEOPLE

PARTS

ACHIEVEMENTS

This year's project is an In vivo production of an antimicrobial peptide regulated by quorum sensing. We designed a synthetic system in E. coli to express an antimicrobial peptide (Cathelicidin LL-37) in vivo only in the presence of a quorum sensing molecule (AI-2) and expel it with an efflux pump in order to act against targeted gastrointestinal pathogens.

We are the team from Facultad de Ciencias, UNAM in Mexico City. Our team brings together students and teachers from all over the country, with various backgrounds and interests.

We submitted several devices mainly to test and use the following parts:
Cathelicidin LL-37: An antimicrobial peptide against bacterial infection.
MarA: A peptide responsible for an efflux pump in E. coli that is related to its resistance to cathelicidin and similar peptides.

The project we developed this year yielded several results. We achieved to characterise and contribute to the iGEM Registry. In this section we present what we think we are our best and main results.

Read the abstract

Meet the team

Read more...

Read more...

News from the lab


Gallery