Team:Ciencias-UNAM/Project

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    <title>Project</title>
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<div id="u5" class="u5"  >
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<div id="u5_rtf"><p style="text-align:center;"><span style="font-family:Museo Slab;font-size:16px;font-weight:normal;font-style:normal;text-decoration:none;color:#4B4F54;">HOME</span></p></div>
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<div id="u6_img" class="u6_normal detectCanvas"></div>
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<div id="u7" class="u7"  >
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<div id="u7_rtf"><p style="text-align:center;"><span style="font-family:Museo Slab;font-size:16px;font-weight:normal;font-style:normal;text-decoration:none;color:#4B4F54;">PROJECT</span></p></div>
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<div id="u9" class="u9"  >
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<div id="u9_rtf"><p style="text-align:center;"><span style="font-family:Museo Slab;font-size:16px;font-weight:normal;font-style:normal;text-decoration:none;color:#4B4F54;">HUMAN PRACTICES </span></p></div>
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<div id="u11" class="u11"  >
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<div id="u11_rtf"><p style="text-align:center;"><span style="font-family:Museo Slab;font-size:16px;font-weight:normal;font-style:normal;text-decoration:none;color:#4B4F54;">TEAM</span></p></div>
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<div id="u13_rtf"><p style="text-align:center;"><span style="font-family:Museo Slab;font-size:16px;font-weight:normal;font-style:normal;text-decoration:none;color:#4B4F54;">SAFETY</span></p></div>
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<div id="u17" class="u17"  >
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<div id="u17_rtf"><p style="text-align:center;"><span style="font-family:Museo Slab;font-size:16px;font-weight:normal;font-style:normal;text-decoration:none;color:#4B4F54;">Overview</span></p></div>
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<div id="u19_rtf"><p style="text-align:center;"><span style="font-family:Museo Slab;font-size:16px;font-weight:normal;font-style:normal;text-decoration:none;color:#4B4F54;">General Design</span></p></div>
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<div id="u21_rtf"><p style="text-align:center;"><span style="font-family:Museo Slab;font-size:16px;font-weight:normal;font-style:normal;text-decoration:none;color:#4B4F54;">Wet Lab</span></p></div>
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<div id="u22_img" class="u22_normal detectCanvas"></div>
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<div id="u23" class="u23"  >
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<div id="u23_rtf"><p style="text-align:center;"><span style="font-family:Museo Slab;font-size:16px;font-weight:normal;font-style:normal;text-decoration:none;color:#4B4F54;">Dry Lab</span></p></div>
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<div id="u25_rtf"><p style="text-align:center;"><span style="font-family:Museo Slab;font-size:16px;font-weight:normal;font-style:normal;text-decoration:none;color:#4B4F54;">Conclusions</span></p></div>
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<div id="u31" class="u31"  >
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<div id="u31_rtf"><p style="text-align:center;"><span style="font-family:Museo Slab;font-size:16px;font-weight:normal;font-style:normal;text-decoration:none;color:#4B4F54;">Bios</span></p></div>
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<div id="u33_rtf"><p style="text-align:center;"><span style="font-family:Museo Slab;font-size:16px;font-weight:normal;font-style:normal;text-decoration:none;color:#4B4F54;">Sponsors</span></p></div>
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<DIV id="u35container" style="position:absolute; left:2px; top:2px; width:179px; height:95px; overflow:visible">
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<div id="u37_rtf"><p style="text-align:center;"><span style="font-family:Museo Slab;font-size:16px;font-weight:normal;font-style:normal;text-decoration:none;color:#4B4F54;">Overview</span></p></div>
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<div id="u38" class="u38_container"  >
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<div id="u38_img" class="u38_normal detectCanvas"></div>
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<div id="u39" class="u39"  >
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<div id="u39_rtf"><p style="text-align:center;"><span style="font-family:Museo Slab;font-size:16px;font-weight:normal;font-style:normal;text-decoration:none;color:#4B4F54;">Journalism</span></p></div>
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<div id="u41_rtf"><p style="text-align:center;"><span style="font-family:Museo Slab;font-size:16px;font-weight:normal;font-style:normal;text-decoration:none;color:#4B4F54;">Interviews</span></p></div>
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<div id="u44"  ></div>
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<DIV id="u45" style="border-style:dotted; border-color:red; visibility:hidden; position:absolute; left:160px; top:20px; width:940px; height:1620px;;" ></div>
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<div id="u46_rtf"><p style="text-align:left;"><span style="font-family:Museo Slab;font-size:14px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;">Home &gt; Project &gt; Overview</span></p></div>
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<DIV id="u47" style="border-style:dotted; border-color:red; visibility:hidden; position:absolute; left:190px; top:200px; width:248px; height:17px;;" ></div>
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<div id="u50_rtf"><p style="text-align:left;"><span style="font-family:Museo Slab;font-size:16px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;">ABSTRACT</span></p><p style="text-align:left;"><span style="font-family:Arial;font-size:14px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;text-decoration:none;">&nbsp;</span></p><p style="text-align:left;"><span style="font-family:Arial;font-size:14px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;">Morbi lacinia neque lorem, non tristique nunc malesuada quis. Morbi nec odio faucibus, hendrerit dolor vitae, viverra purus. Phasellus condimentum scelerisque interdum. Cras id odio a felis pharetra tincidunt eu eu velit. Donec et lorem lacus. Phasellus semper mauris in eleifend interdum. Mauris in viverra neque, ut mollis sem. Aliquam sollicitudin augue vitae augue hendrerit, non gravida nisl fermentum. Suspendisse potenti. Pellentesque habitant morbi tristique senectus et netus et malesuada fames ac turpis egestas. Nulla facilisi. Aenean lorem nisi, pellentesque non venenatis sit amet, tristique vel massa.</span></p><p style="text-align:left;"><span style="font-family:Arial;font-size:14px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;text-decoration:none;">&nbsp;</span></p><p style="text-align:left;"><span style="font-family:Arial;font-size:14px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;">Quisque feugiat cursus sem, vestibulum ornare quam pharetra et. In in fermentum dolor, eu mattis diam. Etiam risus tellus, malesuada quis augue id, sodales vestibulum massa. Integer quis orci erat. Nam nec odio eget magna gravida fringilla. Curabitur dapibus aliquet faucibus. Morbi sed nulla sed odio feugiat vehicula ac rutrum sem.</span></p><p style="text-align:left;"><span style="font-family:Arial;font-size:14px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;text-decoration:none;">&nbsp;</span></p><p style="text-align:left;"><span style="font-family:Arial;font-size:14px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;">Praesent vitae est sit amet lacus placerat cursus. Donec sodales ut arcu a ornare. Mauris sagittis libero velit, sit amet bibendum purus eleifend ut. Praesent a nisi pharetra, imperdiet tortor in, condimentum tellus. Pellentesque interdum neque ut placerat ullamcorper. Ut molestie metus a neque bibendum euismod. Ut a urna vitae nisi ultrices tempor et vel velit. Aenean mattis urna quis turpis rhoncus.</span></p></div>
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<div id="u53_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;">Mauris nec lectus diam. Sed vitae purus imperdiet, iaculis neque eget, euismod est. Lorem ipsum dolor sit amet, consectetur adipiscing elit. Etiam scelerisque, felis in porta mollis, sapien ipsum aliquet nisi, sed tristique nibh nunc facilisis diam. Duis facilisis, orci eu cursus sollicitudin, dui lacus blandit dui, sit amet ullamcorper risus nulla in nisi. Integer a eleifend risus. Aliquam quis eros a metus ultricies congue id id leo. Integer sit amet nunc iaculis, consectetur ipsum et, lobortis justo. Quisque pharetra tellus libero, nec accumsan nisi faucibus quis. Fusce metus orci, egestas vitae hendrerit eget, facilisis id risus. Nam imperdiet, quam sit amet tempus tincidunt, dolor sapien sollicitudin justo, a faucibus nibh justo congue elit.</span></p><p style="text-align:left;"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;text-decoration:none;">&nbsp;</span></p><p style="text-align:left;"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;">Pellentesque habitant morbi tristique senectus et netus et malesuada fames ac turpis egestas. Maecenas ut semper libero. Sed vel risus sit amet nisl tempor fringilla eget vitae mauris. Maecenas eget mauris lobortis, tincidunt ligula a, malesuada mauris. Donec quam nisi, gravida sit amet suscipit eget, ornare ut risus. Cras dui ante, semper at nisi in, volutpat rutrum lectus. Pellentesque orci sem, faucibus venenatis lacus eu, tempus vehicula justo. Lorem ipsum dolor sit amet, consectetur adipiscing elit. Duis molestie elementum magna. Donec faucibus placerat sem, nec lobortis quam pharetra posuere. Lorem ipsum dolor sit amet, consectetur adipiscing elit. Nam convallis aliquam pretium. Nunc eu lectus vitae odio venenatis rutrum ac non est. Morbi congue eros id lobortis fermentum. Aliquam erat metus, interdum mollis laoreet eget, aliquet at mi.</span></p><p style="text-align:left;"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;text-decoration:none;">&nbsp;</span></p><p style="text-align:left;"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;">Aliquam vitae dolor non elit auctor laoreet nec a purus. Fusce lacinia facilisis est nec facilisis. Aliquam ac felis quis mauris ornare semper. Vivamus posuere dui non erat ullamcorper, in semper justo posuere. Donec vitae elit dui. Nulla eget lorem sem. Sed venenatis, magna nec hendrerit commodo, elit tortor fermentum dui, tincidunt tristique dolor nisi non tellus. Donec aliquam.</span></p></div>
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<div id="u54_rtf"><p style="text-align:left;"><span style="font-family:Museo Slab;font-size:16px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;">PROJECT MENU</span></p></div>
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</div>
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<div id="u55_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:14px;font-weight:bold;font-style:normal;text-decoration:none;color:#929EA0;">Overview</span></p></div>
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</div>
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<div id="u56_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:14px;font-weight:bold;font-style:normal;text-decoration:none;color:#929EA0;">General Design</span></p></div>
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</div>
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<div id="u57" class="u57"  >
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<div id="u57_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:14px;font-weight:bold;font-style:normal;text-decoration:none;color:#929EA0;">Wet Lab</span></p></div>
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</div>
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<div id="u58_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:14px;font-weight:normal;font-style:normal;text-decoration:none;color:#929EA0;">BioBricks</span></p></div>
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<div id="u59_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:14px;font-weight:normal;font-style:normal;text-decoration:none;color:#929EA0;">Results</span></p></div>
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<div id="u60_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:14px;font-weight:normal;font-style:normal;text-decoration:none;color:#929EA0;">Characterization</span></p></div>
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<div id="u61_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:14px;font-weight:normal;font-style:normal;text-decoration:none;color:#929EA0;">Protocols</span></p></div>
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<div id="u62_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:14px;font-weight:bold;font-style:normal;text-decoration:none;color:#929EA0;">Dry Lab</span></p></div>
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</div>
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<div id="u63_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:14px;font-weight:normal;font-style:normal;text-decoration:none;color:#929EA0;">Modelling</span></p></div>
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<div id="u64_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:14px;font-weight:bold;font-style:normal;text-decoration:none;color:#929EA0;">Conclusions </span></p></div>
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<div id="u65_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:14px;font-weight:normal;font-style:normal;text-decoration:none;color:#929EA0;">Future Work</span></p></div>
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<div id="u66_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:14px;font-weight:bold;font-style:normal;text-decoration:none;color:#929EA0;">References</span></p></div>
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<b>Abstract</b>
 
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<p>The human peptide LL-37 is a cationic antimicrobial peptide with activity against Gram-negative and Gram-positive bacteria. It has been shown to protect against <i>Helicobacter pylori</i> infection and its related gastritis, besides of inhibiting the growth of other pathogenic bacteria in the gastrointestinal tract such as <i>Salmonella typhimurium</i> and <i>Escherichia coli.</i> Most of the current synthetic expression systems for LL-37 depend on the construction of soluble fusion partners to avoid cytotoxic effects of the antimicrobial peptide in the <i>E. coli</i> host strain. However, these fusion systems require additional cleavage steps using enzymatic or chemical methods, which makes them impossible to express an active LL-37 peptide in vivo. In order to create a resistant host that can export LL-37 to the media, we intend to overexpress the <i>E. coli</i> <i>acrAB</i> and <i>tolC</i> operons, which activate the AcrAB-TolC efflux pump, a mechanism that has been related with resistance to this and similar antimicrobial peptides by expulsion. Our aim is to create a system in which <i>E. coli</i> expels LL-37 only when <i>H. pylori</i> or other pathogenic bacteria, such as <i>S. typhimurium, E. coli</i> or <i>V. cholerae</i>, are present. In order to do this we are using the <i>LsrA</i> promoter, which allows transcription in the presence of AI-2, a molecule produced by bacteria to communicate via <i>quorum-sensing</i>. <i>E. coli</i> also produces AI-2, so we designed an antisense RNA with specific secondary structure to inhibit the translation of LuxS, the enzyme responsible of the production of AI-2 in <i>E. coli</i>. </p>
 
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