Team:Colombia Uniandes/Parameters

Parameters

The second step in the deterministic model is to find the parameters that define the system. There are 3 possible ways to do this:

1. Literature: Many of the genes that we use in iGEM have been characterised before, and the constants that model their behaviour have been found. Sometimes an exact value is not found, so we have to find at least the order of magnitude or a range where the parameter can be found
2. Experiments: This is the most reliable way of knowing the parameters. But in our case we need the to perform de deterministic model first in order to see if the system works as expected in the lab. So the experimental way can only work to check if the parameters were correctly found.
3. Screening: If the parameters we are looking of are not found in literature we proceed to perform a screening of parameters as described in Colombia 2012 Team Wiki

Below is shown how we found the parameters for both of the projects:

Glucocorticoid sensor

As said above the first step to obtain the parameters a literature research was performed. The values for these parameter varied a lot depending on the glucocorticoid detected; as in the lab is planned to test the circuit with Dexamethasone all the parameters were done for this glucocorticoid. Some of the parameters could not be found and their values are in red in the table, an estimated order of magnitude was assessed after reading the literature found

Nickel removal system