Team:Colombia Uniandes/Parts

From 2013.igem.org

(Difference between revisions)
(→Glucocorticoid sensor)
(→Glucocorticoid sensor)
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{| border="1" align="center"
{| border="1" align="center"
|+'''Fusion Checklist*'''
|+'''Fusion Checklist*'''
-
| style="text-align: center;" |pBAP2 + GAL4
+
| style="text-align: center;" |pGAL1 with 1 UAS box+ Terminator
| style="background:#D2D809; text-align: center;"|''Fused''  
| style="background:#D2D809; text-align: center;"|''Fused''  
|-  
|-  
-
| style="text-align: center;" |VP16 + GCR
+
| style="text-align: center;" |pGAL1 with 3 UAS boxes + Terminator
| style="background:#D2D809; text-align: center;"|''Fused''  
| style="background:#D2D809; text-align: center;"|''Fused''  
|-  
|-  
-
| style="text-align: center;" |VP16 + GCR + yeast Terminator
+
| style="text-align: center;" |pGAL1 with 6 UAS boxes + Terminator
-
| style="background:#E30D18; text-align: center;"|''Stand by''
+
| style="background:#D2D809; text-align: center;"|''Fused''  
-
|-
+
|-  
-
| style="text-align: center;" |pBAP2 + GAL4 + VP16 + GCR + yeast Terminator
+
| style="text-align: center;" |pGAL1 with 9 UAS boxes + Terminator
-
| style="background:#E30D18; text-align: center;" |''Stand by''
+
| style="background:#D2D809; text-align: center;"|''Fused''  
-
|-
+
-
| style="text-align: center;" |pGAL1 + mCherry
+
-
| style="background:#D2D809; text-align: center;"|''Fused''
+
|}
|}

Revision as of 00:37, 27 September 2013

Parts

Glucocorticoid sensor

Glucocorticoid sensor

Individual Parts Checklist*
Part Nickname Status
tVP16 A Extracted
GCR HBD B Extracted
pBAP2 C Extracted
GAL4 D Extracted
pGAL1 with 1 UAS box E or E1 Extracted
pGAL1 with 3 UAS boxes E2 Synthesised
pGAL1 with 6 UAS boxes E3 Synthesised
pGAL1 with 9 UAS boxes E4 Synthesised
Yeast Terminator F Synthesised
mCherry G Extracted


Fusion Checklist*
pGAL1 with 1 UAS box+ Terminator Fused
pGAL1 with 3 UAS boxes + Terminator Fused
pGAL1 with 6 UAS boxes + Terminator Fused
pGAL1 with 9 UAS boxes + Terminator Fused

Nickel Removal System

Nickel Removal System

Individual Parts Checklist*
PrcnR In progress
rcnR In progress
PrcnA Extracted
hoxN Extracted
mRFP Extracted


Fusion Checklist*
RBS+hoxN+mRFP+STOP Fused
prcnA+RBS+hoxN Fused
prcnA+RBS+hoxN+mRFP+STOP Fused
prcnR+rcnR+prcnA+RBS+hoxN+mRFP+STOP Stand by



* Updated September 24

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