Team:Colombia Uniandes/Parts

From 2013.igem.org

(Difference between revisions)
(→Glucocorticoid sensor)
(→Glucocorticoid sensor)
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Also, the fluorescence assays for the E*GF parts
Also, the fluorescence assays for the E*GF parts
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[[File:Response1hr.jpg|400px|thumb|left|''' Unsaturated curve where we see how the fluorescence increases after the addition of dexamethasone, a glucocorticoid''']]  
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[[File:Response1hr.jpg|700px|thumb|left|''' Unsaturated curve where we see how the mCherry reporter appears after the addition of 10 uM dexamethasone, a glucocorticoid. Emmision intensity was measured at 607nm''']]  
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[[File:Response3hr.jpg|400px|thumb|right|''' Saturated curve for fluorescence. We can see the different strengths depending on the number of UAS boxes''']]  
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[[File:Response3hr.jpg|700px|thumb|right|''' Saturated curve for fluorescence. After three hours of the addition of 10 uM dexamethasone the reporter (mCherry) reaches its highest signal. We can see the different strengths depending on the number of UAS boxes. Emmision intensity was measured at 607nm''']]  

Revision as of 02:36, 28 September 2013

Parts

Glucocorticoid sensor

Glucocorticoid sensor

Here are the parts sent to the registry:

PartsGC.jpg

You can see the confirmation gels of the parts below:




PCR confirmation: E1GF-E1GF(2)-E2GF-E2GF(2)-WM-E3GF-E3GF(2)-E4GF-E4GF(2)
Co-transformation: WM-E1GF2-E2GF-E3GF1-E3GF2-E4GF-WM- E3GF3-E4GF2-E2GF2
PCR confirmation of the parts cloned in the pSB1C3 backbone using the specifically designed primers 15 and 31 (see primers)


Also, the fluorescence assays for the E*GF parts

Unsaturated curve where we see how the mCherry reporter appears after the addition of 10 uM dexamethasone, a glucocorticoid. Emmision intensity was measured at 607nm
Saturated curve for fluorescence. After three hours of the addition of 10 uM dexamethasone the reporter (mCherry) reaches its highest signal. We can see the different strengths depending on the number of UAS boxes. Emmision intensity was measured at 607nm


Nickel Removal System

Nickel Removal System

Here are the parts sent to the registry:

PartsNi.jpg
Prcna-HoxN and HoxN-RFP


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