Team:Colombia Uniandes/Parts
From 2013.igem.org
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==='''Characterization of reporters'''=== | ==='''Characterization of reporters'''=== | ||
- | To assess the strength of our promoters when induced with Dexamethasone, we performed a fluorometric assay using mCherry as our reporter. | + | To assess the strength of our promoters when induced with Dexamethasone, we performed a fluorometric assay using mCherry as our reporter.Since we don't have our transactivating protein ready yet, we co-transformed our parts with the plasmid pAT7002, which contains a similar inductor, into ''E. coli'' DH10B cells. |
[[File:Response1hr.jpg|700px|thumb|center|''' Unsaturated curve where we see how the mCherry reporter appears after the addition of 10 uM dexamethasone, a glucocorticoid. Emmision intensity was measured at 607nm after excitation at 586nm''']] | [[File:Response1hr.jpg|700px|thumb|center|''' Unsaturated curve where we see how the mCherry reporter appears after the addition of 10 uM dexamethasone, a glucocorticoid. Emmision intensity was measured at 607nm after excitation at 586nm''']] |
Revision as of 03:09, 28 September 2013
Parts
Glucocorticoid sensor
Here are the parts sent to the registry:
Part Name | Registry Number |
E1 | BBa_K1144001 |
E2 | BBa_K1144002 |
E3 | BBa_K1144003 |
E4 | BBa_K1144004 |
E1GF | BBa_K1144005 |
E2GF | BBa_K1144006 |
E3GF | BBa_K1144007 |
E4GF | BBa_K1144008 |
You can see the confirmation gels of the parts below:
Characterization of reporters
To assess the strength of our promoters when induced with Dexamethasone, we performed a fluorometric assay using mCherry as our reporter.Since we don't have our transactivating protein ready yet, we co-transformed our parts with the plasmid pAT7002, which contains a similar inductor, into E. coli DH10B cells.
We also decided to visually inspect our induced transformants. Here two of the images taken using a epifluorescent microscopy with a TRITC filter.