Team:Colombia Uniandes/Parts
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Parts
Glucocorticoid sensor
Here are the parts sent to the registry:
Part Name | Registry Number |
E1 | BBa_K1144001 |
E2 | BBa_K1144002 |
E3 | BBa_K1144003 |
E4 | BBa_K1144004 |
E1GF | BBa_K1144005 |
E2GF | BBa_K1144006 |
E3GF | BBa_K1144007 |
E4GF | BBa_K1144008 |
You can see the confirmation gels of the parts below:
Characterization of reporters
To assess the strength of our promoters when induced with Dexamethasone, we performed a fluorometric assay using mCherry as our reporter.Since we don't have our transactivating protein ready yet, we co-transformed our parts with the pAT7002 vector (Aoyama and Chua, 997, Plant J. 11 , p605-612), which contains a well characterized Glucocorticoid Responsive Element that also uses the GAL4 DNA binding domain into E. coli DH10B cells.
We also decided to visually inspect our induced transformants. Here two of the images taken using a epifluorescent microscopy with a TRITC filter.