Team:DTU-Denmark/Methods

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Methods

Contents


PCR

PCR

PCR-mix

PCR-ramp

PCR-touchdown

PCR-touchUp

PCR-gradient

Colony PCR


USER Cloning

USER Cloning and Transformation

Plasmid isolation

Plasmid isolation by ethanol precipitation

Determining concentration of nitrogen compounds

Experiment 1a -- Toxicity of Nitrite, Nitrate and Ammonium to E. coli

Experiment 2 -- Anaerobic production of nitrous oxide from nitrite

Experiment 4 -- Aerobic consumption of ammonium and hydroxylamine

Other

Visualizing GFP in the periplasm

Preparation of chemically competent cells

Autoclaving

Sparging with N2

Modified DM minimal medium

Calibration of Electrodes

Ammonium colorimetric measurements

Nitrate colorimetric measurements

Nitrite colorimetric measurements

Rehydrating Primers

DpnI treatment

Bioreactor