Team:DTU-Denmark/Notebook/17 July 2013

From 2013.igem.org

Revision as of 14:44, 18 July 2013 by Hezscha (Talk | contribs)


Contents

208


Main purpose


  • Miniprep

Who was in the lab


Gosia, Henrike

Procedure


- miniprep of RFP in pZA21 and cycAX in pZA21

- made gycerol stocks from ON cultures supposedly containing RFP in pZA21 and cycAX in pZA21

- gel of yesterday's PCR (to get AMO with USER endings) -> got the fragment

- purified AMO for USER from gel

- PCR to add His-tags to construct 1 (Sec-GFP-RFP) and construct 2 (TAT-GFP-RFP)

- gel on today's PCR -> construct 1 has no product, construct 2 has an unkown product with length 900 bp