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	# restriction analysis cyc EcoRI		# restriction analysis cyc EcoRI
	# restriction analysis cyc HindIII		# restriction analysis cyc HindIII
		+	# 1 kb ladder

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Revision as of 08:37, 25 July 2013

Contents 1 208 1.1 Main purpose 1.2 Who was in the lab 1.3 Procedure 1.3.1 PCR in order to amplify AMO and HAO with USER primers 1.3.2 gel electrophoresis

208

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Main purpose

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• PCR of AMO and HAO using USER primers and as a template using AMO and HAO extracted from chromosomal DNA with non-uracil primers
• verification of PCRs

Who was in the lab

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Kristian, Gosia

Procedure

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PCR in order to amplify AMO and HAO with USER primers

gel electrophoresis

1% agarose gel was loaded with samples as follows:

1. 1 kb ladder
2. AMO 5uL template, 1
3. AMO 5uL template, 2
4. AMO 10uL template, 1
5. AMO 10uL template, 2
6. HAO 5uL template, 1
7. HAO 5uL template, 2
8. HAO 10uL template, 1
9. HAO 10uL template, 2
10. restriction analysis cyc EcoRI
11. restriction analysis cyc HindIII
12. 1 kb ladder