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Team:DTU-Denmark/Notebook/25 July 2013
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* PCR of AMO and HAO using USER primers and as a template using AMO and HAO extracted from chromosomal DNA with non-uracil primers | * PCR of AMO and HAO using USER primers and as a template using AMO and HAO extracted from chromosomal DNA with non-uracil primers | ||
* verification of PCRs | * verification of PCRs | ||
| - | + | * ON cultures and re-plating of Nir USER transformants from Colony PCR | |
==Who was in the lab== | ==Who was in the lab== | ||
Revision as of 10:49, 25 July 2013
Contents |
208
Main purpose
- PCR of AMO and HAO using USER primers and as a template using AMO and HAO extracted from chromosomal DNA with non-uracil primers
- verification of PCRs
- ON cultures and re-plating of Nir USER transformants from Colony PCR
Who was in the lab
Kristian, Gosia
Procedure
PCR in order to amplify AMO and HAO with USER primers
gel electrophoresis
1% agarose gel was loaded with samples as follows:
- 1 kb ladder
- AMO 5uL template, 1
- AMO 5uL template, 2
- AMO 10uL template, 1
- AMO 10uL template, 2
- HAO 5uL template, 1
- HAO 5uL template, 2
- HAO 10uL template, 1
- HAO 10uL template, 2
- restriction analysis cyc EcoRI
- restriction analysis cyc HindIII
- 1 kb ladder
Results
Conclusion
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