Team:Duke

From 2013.igem.org

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[[File:MainGene.jpg|700px|center]]  
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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|[[Image:Duke_logo.png|900px|center]]
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Project Description
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Following an initial period of growth after the publication of the first synthetic
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gene circuits, development in the field has stalled. This is due in part to the limited
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number of well-characterized parts with desired features. For instance, the function
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of the repressilator and genetic toggle switch both rely on repressible promoters
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with high cooperativity – provided in these cases by multimerization of the
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repressor proteins. The TALE family of transcription factors (TFs) and the CRISPR/
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Cas9 system show promise in expanding the parts list to bind to near-arbitrary
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target sequences, but because they bind to DNA as monomers, promoters under
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their control cannot show cooperativity in their response. 
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It has been shown theoretically and in vivo that repressors binding as monomers
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to multiple binding sites can introduce cooperativity in to a system. With this
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in mind, we are developing an organism-independent approach that leverages
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programmable TFs to create library of independent and orthogonal repressorpromoter pairs with a range of expression parameters (viz. cooperativity, basal
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and maximal expression rate, response time) of potentially unlimited size. It is our
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aim that this approach will enable the field to move beyond toy circuits and begin
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exploring higher-order dynamics.
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|[[Image:team_test.png|border|500px|center|Your team picture]]
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|align="center"|[[Team:Duke | Team Duke]]
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Latest revision as of 00:34, 28 September 2013

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MainGene.jpg