Team:Dundee

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Revision as of 09:21, 7 August 2013

iGEM Dundee 2013 · ToxiMop

The Microcystin Monster

Algal blooms are an ever-growing problem in freshwater systems. At the Beijing Olympics 2008, 10,000 people were hired to clean up the extensive algal bloom in time for the sailing regatta. The main concern is the level of a toxin called microcystin, which is released by cyanobacteria when they die and lyse.

Microcystin, a toxin released by Microcystis aeruginosa, is harmful to mammals due to its ability to latch on to the human protein PP1, thus ceasing its operation. We are exploiting the ability of the human protein phosphatase (PP1) to covalently bind to microcystin, in order to develop a biological mop ‘janitor’ to rid algal bloom water of the toxin.


ToxiMop

Using B. subtilis and E. coli as chassis to express PP1. This will act as a molecular “mop”.

Detector

Engineering the EnvZ and PrkC systems to express GFP or trigger germination in the presence of microcystin.

Moptopus

An electronic sensing device which detects and monitors the state of a freshwater system. It will allow us to predict the likelihood of algal blooms.

Human Practices

Current regulations of measuring water quality may not be appropriate. We organised a political campaign to ignite a nationwide debate.