Team:Dundee/Project
From 2013.igem.org
Kyleharrison (Talk | contribs) |
Kyleharrison (Talk | contribs) |
||
Line 1: | Line 1: | ||
- | <html> | + | <!DOCTYPE html> |
<html lang="en"> | <html lang="en"> | ||
<head> | <head> | ||
Line 9: | Line 9: | ||
<!-- CSS --> | <!-- CSS --> | ||
- | + | <link href="../assets/css/bootstrap.css" rel="stylesheet"> | |
- | <link href=" | + | <link href="../assets/css/style.css" rel="stylesheet"> |
- | <link href=" | + | <style type="text/css"> |
- | + | /* Sticky footer styles | |
+ | -------------------------------------------------- */ | ||
- | + | html, | |
- | + | body { | |
- | + | height: 100%; | |
+ | /* The html and body elements cannot have any padding or margin. */ | ||
} | } | ||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
/* Wrapper for page content to push down footer */ | /* Wrapper for page content to push down footer */ | ||
Line 84: | Line 49: | ||
} | } | ||
} | } | ||
+ | |||
+ | |||
/* Custom page CSS | /* Custom page CSS | ||
Line 101: | Line 68: | ||
</style> | </style> | ||
- | + | <link href="../assets/css/bootstrap-responsive.css" rel="stylesheet"> | |
- | <link href=" | + | |
- | + | ||
<!-- HTML5 shim, for IE6-8 support of HTML5 elements --> | <!-- HTML5 shim, for IE6-8 support of HTML5 elements --> | ||
<!--[if lt IE 9]> | <!--[if lt IE 9]> | ||
- | <script src="/js/html5shiv.js"></script> | + | <script src="../js/html5shiv.js"></script> |
<![endif]--> | <![endif]--> | ||
Line 118: | Line 84: | ||
<body> | <body> | ||
+ | |||
<!-- Part 1: Wrap all page content here --> | <!-- Part 1: Wrap all page content here --> | ||
Line 125: | Line 92: | ||
<div class="navbar navbar-fixed-top"> | <div class="navbar navbar-fixed-top"> | ||
<div class="navbar-inner"> | <div class="navbar-inner"> | ||
- | + | <div class="container"> | |
- | <div class=" | + | |
<button type="button" class="btn btn-navbar" data-toggle="collapse" data-target=".nav-collapse"> | <button type="button" class="btn btn-navbar" data-toggle="collapse" data-target=".nav-collapse"> | ||
<span class="icon-bar"></span> | <span class="icon-bar"></span> | ||
Line 135: | Line 101: | ||
<div class="nav-collapse collapse"> | <div class="nav-collapse collapse"> | ||
<ul class="nav"> | <ul class="nav"> | ||
- | <li class="active"><a href=" | + | <li class="active"><a href="index.html">Home</a></li> |
+ | <li class="active"><a href="Gallery.html">Gallery</a></li> | ||
+ | |||
<li class="dropdown"> | <li class="dropdown"> | ||
<a href="#" class="dropdown-toggle" data-toggle="dropdown">Team <b class="caret"></b></a> | <a href="#" class="dropdown-toggle" data-toggle="dropdown">Team <b class="caret"></b></a> | ||
<ul class="dropdown-menu"> | <ul class="dropdown-menu"> | ||
- | <li><a href=" | + | <li><a href="meettheteam.html">Meet the Team</a></li> |
<li><a href="https://igem.org/Team.cgi?id=1012">Team Information</a></li> | <li><a href="https://igem.org/Team.cgi?id=1012">Team Information</a></li> | ||
- | <li><a href=" | + | <li><a href="#">Acknowledgements</a></li> |
- | <li><a href=" | + | <li><a href="#">Attributions</a></li> |
- | + | ||
<li><a href="#">Contact</a></li> | <li><a href="#">Contact</a></li> | ||
</ul> | </ul> | ||
Line 151: | Line 118: | ||
<a href="#" class="dropdown-toggle" data-toggle="dropdown">Project <b class="caret"></b></a> | <a href="#" class="dropdown-toggle" data-toggle="dropdown">Project <b class="caret"></b></a> | ||
<ul class="dropdown-menu"> | <ul class="dropdown-menu"> | ||
- | <li><a href=" | + | <li><a href="#">Project Overview</a></li> |
<li><a href="#">Notebook</a></li> | <li><a href="#">Notebook</a></li> | ||
<li class="divider"></li> | <li class="divider"></li> | ||
<li class="nav-header">Lab</li> | <li class="nav-header">Lab</li> | ||
- | + | <li class="divider"></li> | |
<li><a href="#">Lab Overview</a></li> | <li><a href="#">Lab Overview</a></li> | ||
<li><a href="#">Detector</a></li> | <li><a href="#">Detector</a></li> | ||
Line 161: | Line 128: | ||
<li class="divider"></li> | <li class="divider"></li> | ||
<li class="nav-header">Modelling </li> | <li class="nav-header">Modelling </li> | ||
- | <li | + | <li class="divider"></li> |
- | <li><a href=" | + | <li><a href="#">Modelling Overview</a></li> |
<li class="divider"></li> | <li class="divider"></li> | ||
<li class="nav-header">Software</li> | <li class="nav-header">Software</li> | ||
+ | <li class="divider"></li> | ||
<li><a href="#">Software Overview</a></li> | <li><a href="#">Software Overview</a></li> | ||
<li><a href="#">Mop-toppus</a></li> | <li><a href="#">Mop-toppus</a></li> | ||
Line 197: | Line 165: | ||
<li class="divider"></li> | <li class="divider"></li> | ||
<li class="nav-header">Media</li> | <li class="nav-header">Media</li> | ||
+ | <li class="divider"></li> | ||
<li><a href="http://www.youtube.com/channel/UCvHOQ9Y1PqKInj6iCwLqTJw/feed?view_as=public">Youtube Channel</a></li> | <li><a href="http://www.youtube.com/channel/UCvHOQ9Y1PqKInj6iCwLqTJw/feed?view_as=public">Youtube Channel</a></li> | ||
<li><a href="#">Graphic Novel</a></li> | <li><a href="#">Graphic Novel</a></li> | ||
Line 203: | Line 172: | ||
<li class="divider"></li> | <li class="divider"></li> | ||
<li class="nav-header">Social Media</li> | <li class="nav-header">Social Media</li> | ||
+ | <li class="divider"></li> | ||
<li><a href="https://www.facebook.com/DundeeiGem2013">Facebook</a></li> | <li><a href="https://www.facebook.com/DundeeiGem2013">Facebook</a></li> | ||
<li><a href="https://twitter.com/DundeeiGEMTeam">Twitter</a></li> | <li><a href="https://twitter.com/DundeeiGEMTeam">Twitter</a></li> | ||
Line 230: | Line 200: | ||
<!-- Title --> | <!-- Title --> | ||
<div class="page-header"> | <div class="page-header"> | ||
- | <h2><b> | + | <h2><b>Project Overview </b> </h2> |
</div> | </div> | ||
<!-- Title End --> | <!-- Title End --> | ||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
<div class="row"> | <div class="row"> | ||
Line 273: | Line 209: | ||
<div class="span6" style="text-align:justify"> | <div class="span6" style="text-align:justify"> | ||
<h2 style="margin-top:-10px;"> The Microcystin Monster </h2> | <h2 style="margin-top:-10px;"> The Microcystin Monster </h2> | ||
- | <p> | + | <p> When conditions become favourable for cyanobacteria in freshwater systems, their population explodes and has devastating effects on the ecosystem by starving other organisms of oxygen. |
+ | This can occur naturally but may also be a result of man’s impact on the environment; e.g. the use of synthetic fertilisers leaching into the water reservoirs such as lakes and ponds. | ||
+ | Cyanobacteria produce many toxins such as microcystin, which are harmful to other organisms, including humans. | ||
+ | <br><br> Microcystin (made by Microcystis aeruginosa and some Planktothrix spp) irreversibly binds to Protein Phosphatase 1 (PP1) in humans, producing detrimental effects in the liver in particular. iGEM Dundee strives to engineer lab bacteria strains to use PP1 as a detector and a mop of microcystin. | ||
+ | We hope to create a microcystin detection system that takes 1 hour to give results, compared to the current 24 hour method. A remote sensor device has also been designed to predict occurrence of algal blooms. | ||
+ | </p> | ||
<br> | <br> | ||
</div> | </div> | ||
Line 279: | Line 220: | ||
<div class="span6"> | <div class="span6"> | ||
- | + | <img id="image-6" src="http://placehold.it/600x300/EB3C8B/000000&text=Something Cool"> | |
- | + | ||
</div> | </div> | ||
Line 289: | Line 229: | ||
<div class="span6" style="text-align:justify"> | <div class="span6" style="text-align:justify"> | ||
- | <h2 style="margin-top:-10px;"> | + | <h2 style="margin-top:-10px;"> Sensor </h2> |
- | <p> | + | <p> At vero eos et accusamus et iusto odio dignissimos ducimus qui blanditiis praesentium voluptatum deleniti atque corrupti quos dolores et quas molestias excepturi sint occaecati cupiditate non provident, similique sunt in culpa qui officia deserunt mollitia animi, id est laborum et dolorum fuga. Et harum quidem rerum facilis est et expedita distinctio.Temporibus autem quibusdam et aut officiis debitis aut rerum necessitatibus saepe eveniet ut et voluptates repudiandae sint et molestiae non recusandae. Itaque earum rerum hic tenetur a sapiente delectus, ut aut reiciendis voluptatibus maiores alias consequatur aut perferendis doloribus asperiores repellat.</p> |
- | + | ||
- | </p> | + | |
</div> | </div> | ||
<div class="span6" style="text-align:justify"> | <div class="span6" style="text-align:justify"> | ||
+ | <h2 style="margin-top:-10px;"> Detector</h2> | ||
+ | <p> | ||
+ | One of our aims is to make a detection system in Bacillus subtilis where spores germinate in response to the presence of microcystin. This uses a modified version of the PrkC receptor (which usually acts as a sensor for the germination of other spores in the surrounding area), substituting PP1 in place of the normal sensor domains. Our idea is that when microcystin binds to the PP1 of the PrkC receptor, this will trigger downstream intracellular effects, resulting in the germination of dormant spores. The awoken bacteria will then produce PP1 (ToxiMop idea) to rid the water of microcystin.</p> | ||
+ | </div> | ||
+ | </div> | ||
- | < | + | <div class="row"> |
- | + | <!-- the main content --> | |
- | + | ||
- | + | ||
- | < | + | <div class="span6"> |
- | < | + | <img id="image-6" src="http://placehold.it/600x300/0699C1/000000&text=Sensor Image"> |
- | + | </div> | |
- | </div> | + | <div class="span6"> |
+ | <img id="image-6" src="http://placehold.it/600x300/C6E546/000000&text=Detector Image"> | ||
+ | </div> | ||
+ | </div><!-- Row End --> | ||
+ | <!-- Row two --> | ||
+ | <div class="row" style="margin-top:20px;"> | ||
+ | |||
+ | <div class="span6" style="text-align:justify"> | ||
+ | <h2 style="margin-top:-10px;"> Toxi-Mop </h2> | ||
+ | <p> | ||
+ | ToxiMop uses the human protein PP1 expressed on the surface of bacteria to act as a biological mop for microcystin. | ||
+ | By covalently binding to protein phosphatase 1 (PP1), microcystin acts as an inhibitor of PP1, a protein which is integral to almost all major signalling pathways in human cells. | ||
+ | We are using cloning techniques to genetically engineer B. subtilis and E. coli to express PP1 on the cell surface so that bacteria can inhibit microcystin in algal blooms, therefore reducing harm to freshwater ecosystems. | ||
+ | </p> | ||
+ | </div> | ||
+ | |||
+ | <div class="span6" style="text-align:justify"> | ||
+ | <h2 style="margin-top:-10px;"> Toxi-Tweet</h2> | ||
+ | <p> | ||
+ | Sed ut perspiciatis unde omnis iste natus error sit voluptatem accusantium doloremque laudantium, totam rem aperiam, eaque ipsa quae ab illo inventore veritatis et quasi architecto beatae vitae dicta sunt explicabo. Nemo enim ipsam voluptatem quia voluptas sit aspernatur aut odit aut fugit, sed quia consequuntur magni dolores eos qui ratione voluptatem sequi nesciunt. Quis autem vel eum iure reprehenderit qui in ea voluptate velit esse quam nihil molestiae consequatur, vel illum qui dolorem eum fugiat quo voluptas nulla pariatur?</p> | ||
+ | </div> | ||
</div> | </div> | ||
- | + | <div class="row"> | |
- | <div class="row" | + | <!-- the main content --> |
- | + | <div class="span6"> | |
- | <div | + | <img id="image-6" src="http://placehold.it/600x300/8066DB/000000&text=Something Cool"> |
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
</div> | </div> | ||
- | |||
- | + | <div class="span6"> | |
- | + | <img id="image-6" src="http://placehold.it/600x300/EB3C8B/000000&text=Something Cool"> | |
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
</div> | </div> | ||
- | + | </div><!-- Row End --> | |
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
<div id="push"></div> | <div id="push"></div> | ||
Line 379: | Line 299: | ||
- | + | ||
+ | <!-- Le javascript | ||
================================================== --> | ================================================== --> | ||
<!-- Placed at the end of the document so the pages load faster --> | <!-- Placed at the end of the document so the pages load faster --> | ||
Line 386: | Line 307: | ||
<script src="http://code.jquery.com/jquery-latest.js"></script> | <script src="http://code.jquery.com/jquery-latest.js"></script> | ||
<script src="http://www.kyleharrison.co.uk/igem/js/bootstrap.min.js"></script> | <script src="http://www.kyleharrison.co.uk/igem/js/bootstrap.min.js"></script> | ||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
</body> | </body> | ||
</html> | </html> |
Revision as of 21:26, 8 July 2013
<!DOCTYPE html>
Project Overview
The Microcystin Monster
When conditions become favourable for cyanobacteria in freshwater systems, their population explodes and has devastating effects on the ecosystem by starving other organisms of oxygen.
This can occur naturally but may also be a result of man’s impact on the environment; e.g. the use of synthetic fertilisers leaching into the water reservoirs such as lakes and ponds.
Cyanobacteria produce many toxins such as microcystin, which are harmful to other organisms, including humans.
Microcystin (made by Microcystis aeruginosa and some Planktothrix spp) irreversibly binds to Protein Phosphatase 1 (PP1) in humans, producing detrimental effects in the liver in particular. iGEM Dundee strives to engineer lab bacteria strains to use PP1 as a detector and a mop of microcystin.
We hope to create a microcystin detection system that takes 1 hour to give results, compared to the current 24 hour method. A remote sensor device has also been designed to predict occurrence of algal blooms.
Sensor
At vero eos et accusamus et iusto odio dignissimos ducimus qui blanditiis praesentium voluptatum deleniti atque corrupti quos dolores et quas molestias excepturi sint occaecati cupiditate non provident, similique sunt in culpa qui officia deserunt mollitia animi, id est laborum et dolorum fuga. Et harum quidem rerum facilis est et expedita distinctio.Temporibus autem quibusdam et aut officiis debitis aut rerum necessitatibus saepe eveniet ut et voluptates repudiandae sint et molestiae non recusandae. Itaque earum rerum hic tenetur a sapiente delectus, ut aut reiciendis voluptatibus maiores alias consequatur aut perferendis doloribus asperiores repellat.
Detector
One of our aims is to make a detection system in Bacillus subtilis where spores germinate in response to the presence of microcystin. This uses a modified version of the PrkC receptor (which usually acts as a sensor for the germination of other spores in the surrounding area), substituting PP1 in place of the normal sensor domains. Our idea is that when microcystin binds to the PP1 of the PrkC receptor, this will trigger downstream intracellular effects, resulting in the germination of dormant spores. The awoken bacteria will then produce PP1 (ToxiMop idea) to rid the water of microcystin.
Toxi-Mop
ToxiMop uses the human protein PP1 expressed on the surface of bacteria to act as a biological mop for microcystin. By covalently binding to protein phosphatase 1 (PP1), microcystin acts as an inhibitor of PP1, a protein which is integral to almost all major signalling pathways in human cells. We are using cloning techniques to genetically engineer B. subtilis and E. coli to express PP1 on the cell surface so that bacteria can inhibit microcystin in algal blooms, therefore reducing harm to freshwater ecosystems.
Toxi-Tweet
Sed ut perspiciatis unde omnis iste natus error sit voluptatem accusantium doloremque laudantium, totam rem aperiam, eaque ipsa quae ab illo inventore veritatis et quasi architecto beatae vitae dicta sunt explicabo. Nemo enim ipsam voluptatem quia voluptas sit aspernatur aut odit aut fugit, sed quia consequuntur magni dolores eos qui ratione voluptatem sequi nesciunt. Quis autem vel eum iure reprehenderit qui in ea voluptate velit esse quam nihil molestiae consequatur, vel illum qui dolorem eum fugiat quo voluptas nulla pariatur?