Team:Edinburgh/Project

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    <li class="side_menu_item" > [[Team:Edinburgh/Project|Overview]] </li>
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    <li class="side_menu_item" >  [[Project_description|Description]]
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        <ul class="side_submenu">
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              <li class="side_menu_subitem"> [[Metal_promoters|Metal promoters]] </li>
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              <li class="side_menu_subitem"> [[Metal_binding|Metal binding]] </li>
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              <li class="side_menu_subitem"> [[Bioethanol|Bioethanol]] </li>
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              <li class="side_menu_subitem"> [[Aggregation|Aggregation]] </li>
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    <li class="side_menu_item" >  [[Team:Edinburgh/Notebook|Notebook]]
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    <li class="side_menu_item" >  [[Team:Edinburgh/Safety|Safety]]
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    <li class="side_menu_item" >  [[Team:Edinburgh/Parts|Parts submitted]] </li>
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    <li class="side_menu_item" >  [[Results|RESULTS]] </li>
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    <li class="side_menu_item" >  [[Team:Edinburgh/Attributions|Attributions]] </li>
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<h3> Results summary </h3>
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* '''We tested [[Team:Edinburgh/Project/Results/Chassis |the growth]] of our experimental strain, <i>Bacillus subtilis 168</i>, under various conditions relevant to the work we are going to perform.'''
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* '''We tested the Kanamycin resistance conferred by [[Team:Edinburgh/Project/Results/Chassis#vector | pTG262]] in <i>B.subtilis</i> and <i>E.coli</i>.
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* '''We created an assembly to demonstrate that [[Team:Edinburgh/Project/Results/Metal promoters Results | Fur box]] is able to repress gene expression upon exposure to high iron concentrations.'''
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* '''We cloned out and characterised a [[Team:Edinburgh/Project/Results/Metal promoters Results#Fur | Fur transcription factor]].'''
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* '''We cloned a Neisserial Ferric binding protein A ([[Team:Edinburgh/Project/Results/Metal_binding_Results|FbpA]]) as a BioBrick.'''
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* '''We generated a [[Team:Edinburgh/Project/Results/Bioethanol_Results| fusion]] of Pyruvate decarboxylase (Pdc) and Alcohol dehydrogense B (AdhB). We characterised it thoroughly and demonstrated that such co-localisation can increase ethanol production at least twofold.'''
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* '''We cloned [[Team:Edinburgh/Project/Results/Aggregation Results|SinR]] and analysed biofilm formation in WT <i>B.subtilis</i>.'''
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== '''Currently working on'' ==
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* '''We had a great summer, and plan to have fun at the Regional Jamboree.'''
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* PDC-AdhB Fusion
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* NRPS cloning
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* Fur protein and Fur box characterisation
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* Bacillus NCIB3610 competence and biofilm characterisation
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Latest revision as of 23:57, 4 October 2013

Results summary

  • We tested the growth of our experimental strain, Bacillus subtilis 168, under various conditions relevant to the work we are going to perform.


  • We tested the Kanamycin resistance conferred by pTG262 in B.subtilis and E.coli.


  • We created an assembly to demonstrate that Fur box is able to repress gene expression upon exposure to high iron concentrations.



  • We cloned a Neisserial Ferric binding protein A (FbpA) as a BioBrick.


  • We generated a fusion of Pyruvate decarboxylase (Pdc) and Alcohol dehydrogense B (AdhB). We characterised it thoroughly and demonstrated that such co-localisation can increase ethanol production at least twofold.


  • We cloned SinR and analysed biofilm formation in WT B.subtilis.


  • We had a great summer, and plan to have fun at the Regional Jamboree.