Team:Edinburgh/Project/Results

From 2013.igem.org

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<h3> Results summary </h3>
<h3> Results summary </h3>
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* ''We tested [[Team:Edinburgh/Project/Results/Chassis the growth]] of our experimental strain, <i>Bacillus subtilis 168</i>, under various conditions relevant to the work we are going to perform.''
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* '''We tested [[Team:Edinburgh/Project/Results/Chassis the growth]] of our experimental strain, <i>Bacillus subtilis 168</i>, under various conditions relevant to the work we are going to perform.'''
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* ''We tested the Kanamycin resistance conferred by pTG262 in <i>B.subtilis</i> and <i>E.coli</i>. We created an assembly to demonstrate that Fur box is able to repress gene expression upon exposure to high iron concentrations.''
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* '''We tested the Kanamycin resistance conferred by [[Team:Edinburgh/Project/Results/Chassis#vector | pTG262]] in <i>B.subtilis</i> and <i>E.coli</i>.  
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* ''We cloned out and characterised a Fur transcription factor.''
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* '''We created an assembly to demonstrate that Fur box is able to repress gene expression upon exposure to high iron concentrations.'''
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* ''We cloned a Neisserial Ferric binding protein A (FbpA) as a BioBrick.''
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* '''We cloned out and characterised a Fur transcription factor.'''
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* ''We generated a fusion of Pyruvate decarboxylase (pdc) and Alcohol dehydrogense B (adhB). We characterised it thoroughly and demonstrated that such co-localisation can increase ethanol production at least twofold.''  
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* '''We cloned a Neisserial Ferric binding protein A (FbpA) as a BioBrick.'''
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* ''We cloned SinR and analysed biofilm formation in WT <i>B.subtilis</i>.''
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* '''We generated a fusion of Pyruvate decarboxylase (pdc) and Alcohol dehydrogense B (adhB). We characterised it thoroughly and demonstrated that such co-localisation can increase ethanol production at least twofold.'''  
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* ''We had a great summer, and plan to have fun at the Regional Jamboree.''
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* '''We cloned SinR and analysed biofilm formation in WT <i>B.subtilis</i>.'''
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* '''We had a great summer, and plan to have fun at the Regional Jamboree.'''
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{{Team:Edinburgh/Footer}}
{{Team:Edinburgh/Footer}}

Revision as of 23:00, 4 October 2013

Results summary


  • We tested the Kanamycin resistance conferred by pTG262 in B.subtilis and E.coli.


  • We created an assembly to demonstrate that Fur box is able to repress gene expression upon exposure to high iron concentrations.


  • We cloned out and characterised a Fur transcription factor.


  • We cloned a Neisserial Ferric binding protein A (FbpA) as a BioBrick.


  • We generated a fusion of Pyruvate decarboxylase (pdc) and Alcohol dehydrogense B (adhB). We characterised it thoroughly and demonstrated that such co-localisation can increase ethanol production at least twofold.


  • We cloned SinR and analysed biofilm formation in WT B.subtilis.


  • We had a great summer, and plan to have fun at the Regional Jamboree.