Team:Edinburgh/Project/Results

From 2013.igem.org

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* '''We created an assembly to demonstrate that Fur box is able to repress gene expression upon exposure to high iron concentrations.'''
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* '''We created an assembly to demonstrate that [[Team:Edinburgh/Project/Results/Metal promoters Results | Fur box]] is able to repress gene expression upon exposure to high iron concentrations.'''
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* '''We cloned out and characterised a Fur transcription factor.'''
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* '''We cloned out and characterised a [[Team:Edinburgh/Project/Results/Metal promoters Results#Fur | Fur transcription factor]].'''

Revision as of 23:05, 4 October 2013

Results summary

  • We tested the growth of our experimental strain, Bacillus subtilis 168, under various conditions relevant to the work we are going to perform.


  • We tested the Kanamycin resistance conferred by pTG262 in B.subtilis and E.coli.


  • We created an assembly to demonstrate that Fur box is able to repress gene expression upon exposure to high iron concentrations.



  • We cloned a Neisserial Ferric binding protein A (FbpA) as a BioBrick.


  • We generated a fusion of Pyruvate decarboxylase (pdc) and Alcohol dehydrogense B (adhB). We characterised it thoroughly and demonstrated that such co-localisation can increase ethanol production at least twofold.


  • We cloned SinR and analysed biofilm formation in WT B.subtilis.


  • We had a great summer, and plan to have fun at the Regional Jamboree.