Team:Evry/Chelator

From 2013.igem.org

(Difference between revisions)
Line 7: Line 7:
<h1>Iron Chelator</h1>
<h1>Iron Chelator</h1>
-
<p>Here we present Fig 1.a and 1.b our plasmid Assembly of enterobactin chelator genes entA-entF into a single operon
+
<p>Here we present Fig 1.a and 1.b our constructions  Assembly of enterobactin chelator genes entA-entF into a single operon
</p>
</p>
<div align='center'>
<div align='center'>
Line 13: Line 13:
</div>
</div>
<p>
<p>
-
<b>Fig 1.a</b> First plasmid containing the enterobactin synthesis genes Ent A, Ent D and Ent F.
+
<b>Fig 1.a</b> First construction containing the enterobactin synthesis genes Ent A, Ent D and Ent F under the control of Lac O operator. Genes fusions were made with flanking restriction sites that are compatible with Biobrick-based cloning.
</p>
</p>

Revision as of 20:41, 4 October 2013

Iron coli project

Iron Chelator

Here we present Fig 1.a and 1.b our constructions Assembly of enterobactin chelator genes entA-entF into a single operon

Fig 1.a First construction containing the enterobactin synthesis genes Ent A, Ent D and Ent F under the control of Lac O operator. Genes fusions were made with flanking restriction sites that are compatible with Biobrick-based cloning.


Fig 1.b Second plasmid containing the enterobactin synthesis genes Ent B, Ent C and Ent E.

NAME FIGURE Description

Lac promoter

Lac Promoter

RBS + EntA

First gene required for enterobactin sythesis

RBS + EntB

Second gene required for enterobactin sythesis

RBS + EntC

Third gene required for enterobactin sythesis

RBS + EntD

Fourth gene required for enterobactin sythesis

RBS + EntE

Fifth gene required for enterobactin sythesis

RBS + EntF

Sixth gene required for enterobactin synthesis

Terminator

Transcription Stop signal

Plasmid

Backbone with ampicillin resistance

Table 1. Genetic elements used to produce the enterobactin siderophore.

Future caracterisation of the construction

The first