http://2013.igem.org/wiki/index.php?title=Team:Evry/Chelator&feed=atom&action=historyTeam:Evry/Chelator - Revision history2024-03-28T20:31:27ZRevision history for this page on the wikiMediaWiki 1.16.5http://2013.igem.org/wiki/index.php?title=Team:Evry/Chelator&diff=315560&oldid=prevCyrpaut at 03:03, 5 October 20132013-10-05T03:03:42Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h1>Iron Chelator</h1></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h1>Iron Chelator</h1></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><h3>First strategy for enterobactin biosynthesis</h3></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Here we present Figure 1 and 2 our constructions which each contain three Lac I regulated enterobactin synthesis genes. Escherichia coli naturally have those genes into a single operon but due to their important lenghts, we decided to divide them into two individual constructions in order to make the cloning easier.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Here we present Figure 1 and 2 our constructions which each contain three Lac I regulated enterobactin synthesis genes. Escherichia coli naturally have those genes into a single operon but due to their important lenghts, we decided to divide them into two individual constructions in order to make the cloning easier.</div></td></tr>
</table>Cyrpauthttp://2013.igem.org/wiki/index.php?title=Team:Evry/Chelator&diff=315553&oldid=prevCyrpaut at 03:03, 5 October 20132013-10-05T03:03:21Z<p></p>
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</table>Cyrpauthttp://2013.igem.org/wiki/index.php?title=Team:Evry/Chelator&diff=314335&oldid=prevEccho at 02:19, 5 October 20132013-10-05T02:19:32Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Even though we tried to simplify the cloning, our many attempts to obtain the constructions failed. We thus investigated every step of our cloning in order to determine why it did not work. We finally assumed that these failures were due to several reasons.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Even though we tried to simplify the cloning, our many attempts to obtain the constructions failed. We thus investigated every step of our cloning in order to determine why it did not work. We finally assumed that these failures were due to several reasons.</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><h3>Future <del class="diffchange diffchange-inline">caracterisation </del>of the construction</h3></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><h3>Future <ins class="diffchange diffchange-inline">caracterization </ins>of the construction</h3></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>In order to characterize our chelator constructions, we intend to use the property of a chemical compound called Chrome Azurol S (CAS). As shown in previous papers(<a href="http://jmbe.asm.org/index.php/jmbe/article/view/249/html_106">Brian C. Louden et al, 2011</a>), it can be used to detect siderophore with a rather simple method called the blue agar CAS assay. Chrome Azurol S molecules produce a blue color when bind to iron and become yellow as the iron is removed by siderophores. Thus, growing our Iron Coli on CAS medium will allow us to confirm the production of siderophore in high iron concentration as we expect the blue medium to turn yellow with our bacteria</p> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p <ins class="diffchange diffchange-inline">id="caracterization_siderophore"</ins>>In order to characterize our chelator constructions, we intend to use the property of a chemical compound called Chrome Azurol S (CAS). As shown in previous papers(<a href="http://jmbe.asm.org/index.php/jmbe/article/view/249/html_106">Brian C. Louden et al, 2011</a>), it can be used to detect siderophore with a rather simple method called the blue agar CAS assay. Chrome Azurol S molecules produce a blue color when bind to iron and become yellow as the iron is removed by siderophores. Thus, growing our Iron Coli on CAS medium will allow us to confirm the production of siderophore in high iron concentration as we expect the blue medium to turn yellow with our bacteria</p> </div></td></tr>
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</table>Ecchohttp://2013.igem.org/wiki/index.php?title=Team:Evry/Chelator&diff=314025&oldid=prevWillR at 02:08, 5 October 20132013-10-05T02:08:11Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Here we present Figure 1 and 2 our constructions which <del class="diffchange diffchange-inline">contain </del>each three Lac I regulated enterobactin synthesis genes. Escherichia coli naturally have those genes into a single operon but due to their important lenghts, we decided to divide them into two <del class="diffchange diffchange-inline">indivudual </del>constructions in order to make the cloning easier.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Here we present Figure 1 and 2 our constructions which each <ins class="diffchange diffchange-inline">contain </ins>three Lac I regulated enterobactin synthesis genes. Escherichia coli naturally have those genes into a single operon but due to their important lenghts, we decided to divide them into two <ins class="diffchange diffchange-inline">individual </ins>constructions in order to make the cloning easier.</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Even though we tried to simplify the cloning, our many <del class="diffchange diffchange-inline">attemps </del>to obtain the constructions failed. We thus investigated every step of our cloning in order to determine why it did not work. We finally assumed that these failures were due to several reasons.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Even though we tried to simplify the cloning, our many <ins class="diffchange diffchange-inline">attempts </ins>to obtain the constructions failed. We thus investigated every step of our cloning in order to determine why it did not work. We finally assumed that these failures were due to several reasons.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>First,the design of the overhangs' parts for the golden gate assembly had not been <del class="diffchange diffchange-inline">thorougly conceived</del>. Indeed, two differents combination in the parts' order were actually possible.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>First,the design of the overhangs' parts for the golden gate assembly had not been <ins class="diffchange diffchange-inline">thoroughly designed</ins>. Indeed, two differents combination in the parts' order were actually possible.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Further more, sequencing results of our plasmids has shown that among the two theorical possibilities, only one of them was obtained in all the clones we have tested. This combination were characterised by a switch in the parts' order, leading to a non functional siderophore production system. Therefore, we came to the conclusion that our functional system, as we engineered it, was probably toxic for our bacteria.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Further more, sequencing results of our plasmids has shown that among the two theorical possibilities, only one of them was obtained in all the clones we have tested. This combination were characterised by a switch in the parts' order, leading to a non functional siderophore production system. Therefore, we came to the conclusion that our functional system, as we engineered it, was probably toxic for our bacteria.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>Thus, we have conceived new cloning approaches. First of all, we chose to extract the different genes of the enterobactin biosynthesis for a new assembly but without refactoring them in order to <del class="diffchange diffchange-inline">keep </del>as much as possible their natural regulation, that we know is <del class="diffchange diffchange-inline">no </del>toxic. Playing it safe, for this approach we also want to divide the different genes on two plasmids.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>Thus, we have conceived new cloning approaches. First of all, we chose to extract the different genes of the enterobactin biosynthesis for a new assembly but without refactoring them in order to <ins class="diffchange diffchange-inline">stick </ins>as much as possible <ins class="diffchange diffchange-inline">to </ins>their natural regulation, that we know is <ins class="diffchange diffchange-inline">not </ins>toxic. Playing it safe, for this approach we also want to divide the different genes on two plasmids.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>Thereafter, we also want to design a single plasmid which would contains the six genes (Fig 3). It is more risky but it would make the co-transformation with the pAceb-<del class="diffchange diffchange-inline">Lac I </del>plasmid easier. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>Thereafter, we also want to design a single plasmid which would contains the six genes (Fig 3). It is more risky but it would make the co-transformation with the pAceb-<ins class="diffchange diffchange-inline">LacI </ins>plasmid easier. </div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>In order to <del class="diffchange diffchange-inline">characterized </del>our chelator constructions, we intend to use the property of a chemical compound called Chrome Azurol S (CAS). As shown in previous papers(<a href="http://jmbe.asm.org/index.php/jmbe/article/view/249/html_106">Brian C. Louden et al, 2011</a>), it can be used to detect siderophore with a rather simple method called the blue agar CAS assay. Chrome Azurol S molecules produce a blue color when bind to iron and become yellow as the iron is removed by siderophores. Thus, growing our Iron Coli on CAS medium will allow us to confirm the production of siderophore in high iron concentration as we expect the blue medium to turn yellow with our bacteria</p> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>In order to <ins class="diffchange diffchange-inline">characterize </ins>our chelator constructions, we intend to use the property of a chemical compound called Chrome Azurol S (CAS). As shown in previous papers(<a href="http://jmbe.asm.org/index.php/jmbe/article/view/249/html_106">Brian C. Louden et al, 2011</a>), it can be used to detect siderophore with a rather simple method called the blue agar CAS assay. Chrome Azurol S molecules produce a blue color when bind to iron and become yellow as the iron is removed by siderophores. Thus, growing our Iron Coli on CAS medium will allow us to confirm the production of siderophore in high iron concentration as we expect the blue medium to turn yellow with our bacteria</p> </div></td></tr>
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</table>WillRhttp://2013.igem.org/wiki/index.php?title=Team:Evry/Chelator&diff=313741&oldid=prevAUDAM at 01:59, 5 October 20132013-10-05T01:59:11Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>In order to characterized our chelator constructions, we intend to use the property of a chemical compound called Chrome Azurol S (CAS). As shown in previous papers(<a href="http://jmbe.asm.org/index.php/jmbe/article/view/249/html_106">Brian C. Louden et al, 2011</a>), it can be used to detect siderophore with a rather simple method called the blue agar CAS assay. Chrome Azurol S molecules produce a blue color when bind to iron and become yellow the iron is removed. Thus, growing our Iron Coli on CAS medium will allow us to confirm the production of siderophore in high iron concentration as we expect the blue medium to turn yellow with our bacteria</p> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>In order to characterized our chelator constructions, we intend to use the property of a chemical compound called Chrome Azurol S (CAS). As shown in previous papers(<a href="http://jmbe.asm.org/index.php/jmbe/article/view/249/html_106">Brian C. Louden et al, 2011</a>), it can be used to detect siderophore with a rather simple method called the blue agar CAS assay. Chrome Azurol S molecules produce a blue color when bind to iron and become yellow <ins class="diffchange diffchange-inline">as </ins>the iron is removed <ins class="diffchange diffchange-inline">by siderophores</ins>. Thus, growing our Iron Coli on CAS medium will allow us to confirm the production of siderophore in high iron concentration as we expect the blue medium to turn yellow with our bacteria</p> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div align="center"> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div align="center"> </div></td></tr>
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</table>AUDAMhttp://2013.igem.org/wiki/index.php?title=Team:Evry/Chelator&diff=313708&oldid=prevBatou at 01:57, 5 October 20132013-10-05T01:57:53Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>In order to characterized our chelator constructions, we intend to use the property of a chemical compound called Chrome Azurol S (CAS). As shown in previous papers(<a href="http://jmbe.asm.org/index.php/jmbe/article/view/249/html_106">Brian C. Louden et al, 2011</a>), it can be used to detect siderophore with a rather simple method called the blue agar CAS assay. Chrome Azurol S molecules produce a blue color when bind to iron and become yellow the iron is removed. Thus, growing our Iron Coli on CAS medium will allow us to confirm the production of siderophore in high iron concentration as we expect the blue medium to turn yellow with our bacteria</p> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>In order to characterized our chelator constructions, we intend to use the property of a chemical compound called Chrome Azurol S (CAS). As shown in previous papers(<a href="http://jmbe.asm.org/index.php/jmbe/article/view/249/html_106">Brian C. Louden et al, 2011</a>), it can be used to detect siderophore with a rather simple method called the blue agar CAS assay. Chrome Azurol S molecules produce a blue color when bind to iron and become yellow the iron is removed. Thus, growing our Iron Coli on CAS medium will allow us to confirm the production of siderophore in high iron concentration as we expect the blue medium to turn yellow with our bacteria</p> </div></td></tr>
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</table>Batouhttp://2013.igem.org/wiki/index.php?title=Team:Evry/Chelator&diff=313696&oldid=prevBatou at 01:57, 5 October 20132013-10-05T01:57:21Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>In order to characterized our chelator constructions, we intend to use the property of a chemical compound called Chrome Azurol S (CAS). As shown in previous papers(<a href="http://jmbe.asm.org/index.php/jmbe/article/view/249/html_106">Brian C. Louden et al, 2011</a>), it can be used to detect siderophore with a rather simple method called the blue agar CAS assay. Chrome Azurol S molecules produce a blue color when bind to iron and become yellow the iron is removed. Thus, growing our Iron Coli on CAS medium will allow us to confirm the production of siderophore in high iron concentration as we expect the blue medium to turn yellow with our bacteria</p> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>In order to characterized our chelator constructions, we intend to use the property of a chemical compound called Chrome Azurol S (CAS). As shown in previous papers(<a href="http://jmbe.asm.org/index.php/jmbe/article/view/249/html_106">Brian C. Louden et al, 2011</a>), it can be used to detect siderophore with a rather simple method called the blue agar CAS assay. Chrome Azurol S molecules produce a blue color when bind to iron and become yellow the iron is removed. Thus, growing our Iron Coli on CAS medium will allow us to confirm the production of siderophore in high iron concentration as we expect the blue medium to turn yellow with our bacteria</p> </div></td></tr>
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</table>Batouhttp://2013.igem.org/wiki/index.php?title=Team:Evry/Chelator&diff=313584&oldid=prevAUDAM at 01:53, 5 October 20132013-10-05T01:53:41Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Here we present <del class="diffchange diffchange-inline">Fig </del>1 and 2 our constructions which contain each three Lac I regulated enterobactin synthesis genes. Escherichia coli naturally have those genes into a single operon but due to their important lenghts, we decided to divide them into two indivudual constructions in order to make the cloning easier.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Here we present <ins class="diffchange diffchange-inline">Figure </ins>1 and 2 our constructions which contain each three Lac I regulated enterobactin synthesis genes. Escherichia coli naturally have those genes into a single operon but due to their important lenghts, we decided to divide them into two indivudual constructions in order to make the cloning easier.</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><b><del class="diffchange diffchange-inline">Fig </del>1</b> First construction containing the Lac I regulated enterobactin synthesis genes Ent A, Ent D and Ent F. Genes fusions were made with flanking restriction sites that are compatible with Biobrick-based cloning.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><b><ins class="diffchange diffchange-inline">Figure </ins>1</b> First construction containing the Lac I regulated enterobactin synthesis genes Ent A, Ent D and Ent F. Genes fusions were made with flanking restriction sites that are compatible with Biobrick-based cloning.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><b><del class="diffchange diffchange-inline">Fig </del>2</b> Second construction containing the Lac I regulated enterobactin synthesis genes Ent B, Ent C and Ent E. Genes fusions were made with flanking restriction sites that are compatible with Biobrick-based cloning. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><b><ins class="diffchange diffchange-inline">Figure </ins>2</b> Second construction containing the Lac I regulated enterobactin synthesis genes Ent B, Ent C and Ent E. Genes fusions were made with flanking restriction sites that are compatible with Biobrick-based cloning. </div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <b>Figure <del class="diffchange diffchange-inline">3 </del>: </b>Exemple of a CAS plate (Brian C. Louden et al., 2011)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <b>Figure <ins class="diffchange diffchange-inline">4 </ins>: </b>Exemple of a CAS plate (Brian C. Louden et al., 2011)</div></td></tr>
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</table>AUDAMhttp://2013.igem.org/wiki/index.php?title=Team:Evry/Chelator&diff=313557&oldid=prevAUDAM at 01:52, 5 October 20132013-10-05T01:52:46Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h3>Future caracterisation of the construction</h3></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h3>Future caracterisation of the construction</h3></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>In order to characterized our chelator constructions, we intend to use the property of a chemical compound called Chrome <del class="diffchange diffchange-inline">azurol </del>S. As shown in previous papers(<a href="http://jmbe.asm.org/index.php/jmbe/article/view/249/html_106">Brian C. Louden et al, 2011</a>), it can be used <del class="diffchange diffchange-inline">for a </del>siderophore <del class="diffchange diffchange-inline">detection based on </del>simple Chrome <del class="diffchange diffchange-inline">azurol </del>S with <del class="diffchange diffchange-inline">agar plates</del>. <del class="diffchange diffchange-inline"> </del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>In order to characterized our chelator constructions, we intend to use the property of a chemical compound called Chrome <ins class="diffchange diffchange-inline">Azurol </ins>S <ins class="diffchange diffchange-inline">(CAS)</ins>. As shown in previous papers(<a href="http://jmbe.asm.org/index.php/jmbe/article/view/249/html_106">Brian C. Louden et al, 2011</a>), it can be used <ins class="diffchange diffchange-inline">to detect </ins>siderophore <ins class="diffchange diffchange-inline">with a rather </ins>simple <ins class="diffchange diffchange-inline">method called the blue agar CAS assay. </ins>Chrome <ins class="diffchange diffchange-inline">Azurol </ins>S <ins class="diffchange diffchange-inline">molecules produce a blue color when bind to iron and become yellow the iron is removed. Thus, growing our Iron Coli on CAS medium will allow us to confirm the production of siderophore in high iron concentration as we expect the blue medium to turn yellow </ins>with <ins class="diffchange diffchange-inline">our bacteria</p> </ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div></<del class="diffchange diffchange-inline">p</del>> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <div class="captionedPicture" style="width:100%;"></ins></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins><<ins class="diffchange diffchange-inline">img alt="HCversion" src="https://static.igem.org/mediawiki/2013/1/14/249-2331-1-PB.gif" class="Picture"</ins>/></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <b>Figure 3 : </b>Exemple of a CAS plate (Brian C. Louden et al., 2011)</ins></div></td></tr>
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</table>AUDAMhttp://2013.igem.org/wiki/index.php?title=Team:Evry/Chelator&diff=313152&oldid=prevAUDAM at 01:39, 5 October 20132013-10-05T01:39:42Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h3>Future caracterisation of the construction</h3></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h3>Future caracterisation of the construction</h3></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>In order to characterized our chelator constructions, we intend to use the property of a chemical compound called Chrome azurol S. As shown in previous papers(<a href="http://<del class="diffchange diffchange-inline">www</del>.<del class="diffchange diffchange-inline">ncbi</del>.<del class="diffchange diffchange-inline">nlm.nih</del>.<del class="diffchange diffchange-inline">gov</del>/<del class="diffchange diffchange-inline">pubmed</del>/<del class="diffchange diffchange-inline">?term=Detection+of+siderophore+production+from+several+fungi+and+bacteria+by+a+modi%EF%AC%81cation+of+chrome+azurol+S+(CAS)+agar+plate+assay</del>"><del class="diffchange diffchange-inline">Adriane M.F</del>. et al, <del class="diffchange diffchange-inline">1999</del></a>) </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>In order to characterized our chelator constructions, we intend to use the property of a chemical compound called Chrome azurol S. As shown in previous papers(<a href="http://<ins class="diffchange diffchange-inline">jmbe</ins>.<ins class="diffchange diffchange-inline">asm</ins>.<ins class="diffchange diffchange-inline">org/index</ins>.<ins class="diffchange diffchange-inline">php</ins>/<ins class="diffchange diffchange-inline">jmbe</ins>/<ins class="diffchange diffchange-inline">article/view/249/html_106</ins>"><ins class="diffchange diffchange-inline">Brian C</ins>. <ins class="diffchange diffchange-inline">Louden </ins>et al, <ins class="diffchange diffchange-inline">2011</ins></a>)<ins class="diffchange diffchange-inline">, it can be used for a siderophore detection based on simple Chrome azurol S with agar plates. </ins></div></td></tr>
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</table>AUDAM