Team:Evry/Chelator

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Iron coli project

Iron Chelator

Here we present Fig 1.a and 1.b our plasmid Assembly of enterobactin chelator genes entA-entF into a single operon

Fig 1.a First plasmid containing the enterobactin synthesis genes Ent A, Ent D and Ent F.


Fig 1.b Second plasmid containing the enterobactin synthesis genes Ent B, Ent C and Ent E.

NAME FIGURE Description

Lac promoter

Lac Promoter

RBS + EntA

First gene required for enterobactin sythesis

RBS + EntB

Second gene required for enterobactin sythesis

RBS + EntC

Third gene required for enterobactin sythesis

RBS + EntD

Fourth gene required for enterobactin sythesis

RBS + EntE

Fifth gene required for enterobactin sythesis

RBS + EntF

Sixth gene required for enterobactin synthesis

Terminator

Transcription Stop signal

Plasmid

Backbone with ampicillin resistance

Table 1. Genetic elements used to produce the enterobactin siderophore.

Future caracterisation of the construction

The first