Team:Glendale CC AZ/Protocols/RestrictionDigest

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== Restriction Digest Protocol ==
== Restriction Digest Protocol ==

Revision as of 07:53, 18 August 2013

Restriction Digest Protocol

Protocol can be found at: http://parts.igem.org/Help:Protocols/Restriction_Digest


Materials 

  -Ice and bucket/container
  -Part A (Purified DNA, > 16ng/µl)
  -Part B (Purified DNA, > 16ng/µl)
  -Linearized plasmid backbone (25ng/µl)
  -dH2O
  -NEB Buffer 2, 3.1,or Ecor1
  -BSA (Not needed with 3.1 buffer)
  -Restriction Enzymes: EcoRI, SpeI, XbaI, PstI
  -Thermal cycler


Procedure

Retrieved from "http://2013.igem.org/Team:Glendale_CC_AZ/Protocols/RestrictionDigest"