"
Team:Glendale CC AZ/Protocols/Transformation
From 2013.igem.org
(Difference between revisions)
E.Soboslay (Talk | contribs) |
E.Soboslay (Talk | contribs) |
||
| Line 8: | Line 8: | ||
== Materials == | == Materials == | ||
| - | + | -Resuspended DNA | |
| + | -Competent cells (50ul per transformation) | ||
| + | -Ice | ||
| + | -2ml tube (1 per a transformation) | ||
| + | -42ºC water bath | ||
| + | -SOC media | ||
| + | -Petri dishes with LB agar and appropriate antibiotic (2 per transformation) | ||
| + | -Spreader | ||
| + | -37ºC incubator | ||
| + | -10pg/ul RFP Control (pSB1A3 w/ BBa_J04450) | ||
== Procedure == | == Procedure == | ||
Revision as of 21:25, 11 August 2013
Transformation Protocol
Protocol from: http://parts.igem.org/Help:Protocols/Transformation
Materials
-Resuspended DNA -Competent cells (50ul per transformation) -Ice -2ml tube (1 per a transformation) -42ºC water bath -SOC media -Petri dishes with LB agar and appropriate antibiotic (2 per transformation) -Spreader -37ºC incubator -10pg/ul RFP Control (pSB1A3 w/ BBa_J04450)
