Team:Goettingen/Team/Reporter
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<p><b>Figure 1a:</b> When there is no c-di-AMP present, the repressor gene DarR cannot bind to its binding site(operator sequence). The transciption starts and leads to the expression of the reporter gene. This reporter gene could be GFP, or other widely used reporter gene.</p> | <p><b>Figure 1a:</b> When there is no c-di-AMP present, the repressor gene DarR cannot bind to its binding site(operator sequence). The transciption starts and leads to the expression of the reporter gene. This reporter gene could be GFP, or other widely used reporter gene.</p> | ||
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<p><b>Figure 1b: </b> When there is c-di-AMP present, the c-di-AMP can act as ligand of the repressor gene DarR, activating tis function. DarR binds to its binding site, blocking the RNA polymerase, and therefore disrupt transcription. Then we are supposed to see no signal or reduced signal.</p></div> | <p><b>Figure 1b: </b> When there is c-di-AMP present, the c-di-AMP can act as ligand of the repressor gene DarR, activating tis function. DarR binds to its binding site, blocking the RNA polymerase, and therefore disrupt transcription. Then we are supposed to see no signal or reduced signal.</p></div> | ||
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Revision as of 19:38, 26 September 2013