Team:Goettingen/Team/Reporter
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Ideally, this c-di-AMP-sensing system works in the following way (Fig. 1.3): Without c-di-AMP, DarR is not bound to the DarR operator, so GFP is expressed resulting in green fluorescing cells. With c-di-AMP, DarR can bind to its binding sequence, repressing gfp transcription, leading to non-fluorescent cells. In the same way, the system might react to compounds similar to c-di-AMP. It can therefore be used to screen for compounds similar to c-di-AMP. It can be used in a large throughput scale, as well. This way, the DarR reporter system will make it possible to find inhibitors and competitors which might be used as antibiotics. | Ideally, this c-di-AMP-sensing system works in the following way (Fig. 1.3): Without c-di-AMP, DarR is not bound to the DarR operator, so GFP is expressed resulting in green fluorescing cells. With c-di-AMP, DarR can bind to its binding sequence, repressing gfp transcription, leading to non-fluorescent cells. In the same way, the system might react to compounds similar to c-di-AMP. It can therefore be used to screen for compounds similar to c-di-AMP. It can be used in a large throughput scale, as well. This way, the DarR reporter system will make it possible to find inhibitors and competitors which might be used as antibiotics. | ||
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<p>Reference:</p> | <p>Reference:</p> | ||
1. <a href="http://www.jbc.org/content/288/5/3085" style="color:#303030" >Zhang <i>et.</i>al.(2013)DarR, a TetR-like Transcriptional Factor, Is a Cyclic Di-AMP-responsive Repressor in <i>Mycobacterium smegmatis, J. Biol. Chem. </i> 288:3085–3096</a> | 1. <a href="http://www.jbc.org/content/288/5/3085" style="color:#303030" >Zhang <i>et.</i>al.(2013)DarR, a TetR-like Transcriptional Factor, Is a Cyclic Di-AMP-responsive Repressor in <i>Mycobacterium smegmatis, J. Biol. Chem. </i> 288:3085–3096</a> |
Revision as of 09:22, 1 October 2013