Team:Goettingen/Team/Reporter
From 2013.igem.org
(→DarR reporter system (part BBa_K1045017):) |
(→DarR reporter system (part BBa_K1045017):) |
||
Line 57: | Line 57: | ||
<html><a href="https://static.igem.org/mediawiki/2013/e/ea/Goe-rt-cloningSteps.png" target="_blank"><img src="https://static.igem.org/mediawiki/2013/e/ea/Goe-rt-cloningSteps.png" width="750"/></a></html> | <html><a href="https://static.igem.org/mediawiki/2013/e/ea/Goe-rt-cloningSteps.png" target="_blank"><img src="https://static.igem.org/mediawiki/2013/e/ea/Goe-rt-cloningSteps.png" width="750"/></a></html> | ||
+ | ''Fig. 1.1. The DarR reporter system was assembled step by step in pSB1C3. If a certain biobrick of a specific cloning step was used as a vector, insert or derived from hybridization oligos or PCR is indicated in blue. The part numbers of the intermediates are given in black. The orientation of the arrows corresponds to the orientation of the element in the vector.'' | ||
+ | |||
+ | <html><a href="https://static.igem.org/mediawiki/2013/6/6c/Goe-rt-assemblingStrategy.png" target="_blank"><img src="https://static.igem.org/mediawiki/2013/6/6c/Goe-rt-assemblingStrategy.png" width="750" /></a></html> | ||
+ | |||
+ | ''Figure 1.2: The final DarR reporter system consisted of two expression units in diverging orientation.'' | ||
+ | |||
+ | <html><a href="https://static.igem.org/mediawiki/2013/f/f6/Goe-rt-DarRsystem.png" target="_blank"><img src="https://static.igem.org/mediawiki/2013/f/f6/Goe-rt-DarRsystem.png" width="750"></a></html> | ||
+ | |||
+ | |||
+ | ''Figure 1.3: Without c-di-AMP, E. coli cells expressing the DarR reporter system, will fluoresce green. With c-di-AMP, they will not fluoresce.'' | ||
<html> | <html> | ||
Line 64: | Line 74: | ||
<br /> | <br /> | ||
</html> | </html> | ||
- | + | ==Riboswitch reporter system (part BBa_K1045002):== | |
=== === | === === | ||
<html> | <html> |
Revision as of 09:34, 1 October 2013