Team:Goettingen/Team/Reporter

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The beast and its Achilles heel:

 A novel target to fight multi-resistant pathogenic bacteria



Reporter Team

The activity of transcriptional regulators can be easily detected by monitoring the activity of a promoter that is fused to a reporter gene. Recently, in Mycobacterium smegmatis, a transcriptional repressor (DarR) was identified that is able to bind to a specific DNA sequence upon association with c-di-AMP. This led us to the idea of developing a screening system for potential drugs interfering with c-di-AMP biofunction. While many Gram-positive bacteria require c-di-AMP for their growth, this molecule is not synthesized by the Gram-negative bacterium E. coli. Thus, we intend to build a reporter system that is composed of existing and of novel biobricks that will be developed during the project (Figure 1).


Reference

1. Zhang et al. (2013) DarR, a TetR-like transcriptional factor, Is a cyclic di-AMP-responsive repressor in Mycobacterium smegmatis, J. Biol. Chem. 288:3085–3096

 

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