Team:Goettingen/Team/Reporter

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The beast and its Achilles heel:

 A novel target to fight multi-resistant pathogenic bacteria



Reporter Team

The activity of transcriptional regulators can be easily detected by monitoring the activity of a promoter that is fused to a reporter gene such as the green fluorescent protein (GFP). Recently, in Mycobacterium smegmatis, the transcriptional repressor DarR was identified (Zhang et al. 2013). DarR is capable of binding to a specific DNA sequence upon association with c-di-AMP. This led us to the idea of developing a powerful screening system to facilitate the identification of potential drugs interfering with c-di-AMP biofunction. While many Gram-positive bacteria (also pathogenic bacteria that cause severe human diseases) require c-di-AMP for growth, c-di-AMP is not synthesized by the Gram-negative bacterium Escherichia coli. Therefore, we intend to build a reporter system that is composed of existing and of novel biobricks that will be developed during the project (Figure 1). As E. coli does not require c-di-AMP for growth the screening system can be used to sort out those molecules from a drug library that interfere with general cellular processes (i.e. replication). By contrast, the system is suitable to identify compounds that specifically interfere with the signaling function of c-di-AMP.


Reference

1. Zhang et al. (2013) DarR, a TetR-like transcriptional factor, Is a cyclic di-AMP-responsive repressor in Mycobacterium smegmatis, J. Biol. Chem. 288:3085–3096

 

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