Team:Grenoble-EMSE-LSU

From 2013.igem.org

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                                 <p>Our system utilizes <em>Escherichia coli (E. coli)</em> bacteria that produce the <a href="https://2013.igem.org/Team:Grenoble-EMSE-LSU/Project/Biology#KillerRed">photosensitizing protein KillerRed</a> (KR). When illuminated with light, the fluorescent protein KR (580/630nm) produces Reactive Oxygen Species (ROS). These species irreversibly damage cell proteins, membranes, and DNA, ultimately leading to cell death <a href="#ref_home">[2]</a>. Bacterial growth is monitored by measuring the fluorescence of cells containing KillerRed, and population control can be achieved by modulating the amount of ROS produced inside the bacteria with light stimulation. Since the amount of ROS produced is closely related to the amount of intracellular KillerRed, a photosensitive system <a href="#ref_home">[3-4]</a> was developed to regulate the concentration of this protein.<br><br>
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                                 <p>Our system utilizes <em>Escherichia coli (E. coli)</em> bacteria that produce the <a href="https://2013.igem.org/Team:Grenoble-EMSE-LSU/Project/Biology#KillerRed">photosensitizing protein KillerRed</a>. When illuminated with light, the fluorescent protein KillerRed (580/630nm) produces Reactive Oxygen Species (ROS). These species irreversibly damage cell proteins, membranes, and DNA, ultimately leading to cell death <a href="#ref_home">[2]</a>. Bacterial growth is monitored by measuring the fluorescence of cells containing KillerRed, and population control can be achieved by modulating the amount of ROS produced inside the bacteria with light stimulation. Since the amount of ROS produced is closely related to the amount of intracellular KillerRed, a photosensitive system <a href="#ref_home">[3-4]</a> was developed to regulate the concentration of this protein.<br><br>
                                 We have built a mathematical model based on the results given by our biological experiments, that can help <a href="https://2013.igem.org/Team:Grenoble-EMSE-LSU/Project/Modelling/Building">predict the amount of living cells within our culture</a> and their growth rate in a specific set of experimental conditions. These conditions depend on: light intensity, illumination time, and concentration of intracellular KillerRed protein. The model was further implemented on a, Arduino microcontroller, which drives our electronic system TalkE'Coli. This device, equipped with a white light source, a set of filters and a photodiode, can then be used to regulate living cell population and growth to any arbitrary level within natural limits.<br><br>
                                 We have built a mathematical model based on the results given by our biological experiments, that can help <a href="https://2013.igem.org/Team:Grenoble-EMSE-LSU/Project/Modelling/Building">predict the amount of living cells within our culture</a> and their growth rate in a specific set of experimental conditions. These conditions depend on: light intensity, illumination time, and concentration of intracellular KillerRed protein. The model was further implemented on a, Arduino microcontroller, which drives our electronic system TalkE'Coli. This device, equipped with a white light source, a set of filters and a photodiode, can then be used to regulate living cell population and growth to any arbitrary level within natural limits.<br><br>
                                 </p>
                                 </p>

Revision as of 01:54, 5 October 2013

Grenoble-EMSE-LSU, iGEM


Grenoble-EMSE-LSU, iGEM

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