Team:Grenoble-EMSE-LSU

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                                 Our system involves Escherichia Coli (E. coli) bacteria, producing the photosensitizing protein KillerRed. Upon light irradiation, this fluorescent protein (580/630 nm) produces Reactive Oxygen Species (ROS) which irreversibly damage cell proteins, membranes and DNA, leading to cell death [2]. Bacterial growth is here followed by monitoring the KillerRed-expressing cell red fluorescence and can be controlled by modulating the amount of ROS produced inside the bacteria, using white light stimulations. Since the amount of ROS produced, and thus the cytotoxicity, is closely related to the concentration in intracellular KillerRed, a photosensitive system enabling to control this protein level inside the cell was also developed.<br><br>
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                                 Our system involves <em>Escherichia coli (E. coli)</em> bacteria, producing the photosensitizing protein KillerRed. Upon light irradiation, this fluorescent protein (580/630 nm) produces Reactive Oxygen Species (ROS) which irreversibly damage cell proteins, membranes and DNA, leading to cell death [2]. Bacterial growth is monitored by the red fluorescence of KillerRed-expressing cells and can be controlled by modulating the amount of ROS produced inside the bacteria, using white light stimulations. Since the amount of ROS produced, and thus the cytotoxicity, is closely related to the concentration in intracellular KillerRed, a photosensitive system [3] enabling to control this protein level inside the cell was also developed.<br><br>
                                 The results given by the biological experiments has enabled us to build a mathematical model that can help predicting the amount of living cells within our culture and their growth rate, in a specific set of experimental conditions (light intensity, illumination time, concentration in intracellular KillerRed protein). This model was further implemented on a microcontroller, driving our electronic system Talk’E. Coli. This device, equipped with different light sources and a photodiode, can then be used for regulating cell population and growth to any arbitrary level within natural limits.<br><br>
                                 The results given by the biological experiments has enabled us to build a mathematical model that can help predicting the amount of living cells within our culture and their growth rate, in a specific set of experimental conditions (light intensity, illumination time, concentration in intracellular KillerRed protein). This model was further implemented on a microcontroller, driving our electronic system Talk’E. Coli. This device, equipped with different light sources and a photodiode, can then be used for regulating cell population and growth to any arbitrary level within natural limits.<br><br>
                                 <em>References:</em><br>
                                 <em>References:</em><br>
                                 [1] L. Elsgaard and D. Prieur, Hydrothermal vents in Lake Tanganyika harbor spore-forming thermophiles with extremely rapid growth, <em>Journal of Great Lakes Research</em>, March 2011.<br>
                                 [1] L. Elsgaard and D. Prieur, Hydrothermal vents in Lake Tanganyika harbor spore-forming thermophiles with extremely rapid growth, <em>Journal of Great Lakes Research</em>, March 2011.<br>
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                                 [2] M.E. Bulina et al., A genetically encoded photosensitizer, <em>Nature Biotechnology</em>, January 2006.
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                                 [2] M.E. Bulina et al., A genetically encoded photosensitizer, <em>Nature Biotechnology</em>, January 2006.<br>
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                                [3]
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Revision as of 09:07, 9 August 2013

Grenoble-EMSE-LSU, iGEM


Grenoble-EMSE-LSU, iGEM

Retrieved from "http://2013.igem.org/Team:Grenoble-EMSE-LSU"