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Team:Grenoble-EMSE-LSU
From 2013.igem.org
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Light allows for precise machine-to-cell and cell-to-machine communication in both time and space and was thus elected to <a>interface a biological cell culture to our electronic device</a>.<br> | Light allows for precise machine-to-cell and cell-to-machine communication in both time and space and was thus elected to <a>interface a biological cell culture to our electronic device</a>.<br> | ||
| + | <figure> | ||
<img src="https://static.igem.org/mediawiki/2013/9/9a/1ere_version_image.png" alt="Project overview" width="600px" /> | <img src="https://static.igem.org/mediawiki/2013/9/9a/1ere_version_image.png" alt="Project overview" width="600px" /> | ||
| + | <figcaption>Overview on Light Automated Cell Control (Lac²).<br> | ||
| + | Source: Carpentier et al., Structural Basis for the Phototoxicity of the Fluorescent Protein KillerRed, <em>FEBS Letters</em> 583.17 (2009): 2839-842</figcaption> | ||
| + | </figure> | ||
Our system utilizes <em>Escherichia coli (E. coli)</em> bacteria that are producing the <a>photosensitizing protein KillerRed</a> (KR). When illuminated with light, the KR fluorescent protein (580/630nm) produces <a>Reactive Oxygen Species</a> (ROS). These species irreversibly damage cell proteins, membranes, and DNA, ultimately leading to cell death [2]. Bacterial growth is monitored by measuring the fluorescence of cells containing KillerRed, and population control can be achieved by modulating the amount of ROS produced inside the bacteria with light stimulation. Since the amount of ROS produced is closely related to the amount of intracellular KillerRed, a photosensitive system [3-4] was developed to regulate the concentration of this protein.<br><br> | Our system utilizes <em>Escherichia coli (E. coli)</em> bacteria that are producing the <a>photosensitizing protein KillerRed</a> (KR). When illuminated with light, the KR fluorescent protein (580/630nm) produces <a>Reactive Oxygen Species</a> (ROS). These species irreversibly damage cell proteins, membranes, and DNA, ultimately leading to cell death [2]. Bacterial growth is monitored by measuring the fluorescence of cells containing KillerRed, and population control can be achieved by modulating the amount of ROS produced inside the bacteria with light stimulation. Since the amount of ROS produced is closely related to the amount of intracellular KillerRed, a photosensitive system [3-4] was developed to regulate the concentration of this protein.<br><br> | ||
Revision as of 12:55, 12 August 2013
