Team:Grenoble-EMSE-LSU/Documentation/Notebook/June

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<h1>June</h1>
<h1>June</h1>
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                                          <h2>Week 1</h2>
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                                          <h3>Monday</h3>
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                                              <h3>Tuesday</h3>
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                                              <h3>Wednesday</h3>
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                                              <h3>Thursday</h3>
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                                              <h3>Friday</h3>
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                                                <h4>Team meeting</h4>
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                                          <h2>Week 2</h2>
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<p>
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Little Summary with Keywords
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</p>
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                                              <h3>Monday</h3>
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                                                <h4>Construction of the pQE30::KillerRed vector</h4>
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<p>
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Incubated a 50mL overnight culture of E. coli M15[pRep4] cells (Qiagen, Venlo, Netherlands), containing the pQE30::&alpha;SNAP vector, in preparation for midiprep.
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</p>
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                                              <h3>Tuesday</h3>
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                                                <h4>Construction of the pQE30::KillerRed vector</h4>
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<p>
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Midi-prepped pQE30::&alpha;SNAP. The DNA sample concentration was only of 10 ng/µL. The experiment has to be re performed. <br/> <br/>
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Incubated a 10mL overnight culture of E. coli M15[pRep4-pQE30::&alpha;SNAP] cells, in preparation for miniprep.
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</p>
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                                              <h3>Wednesday</h3>
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                                                <h4>Construction of the pQE30::KillerRed vector</h4>
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<p>
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Mini-prepped pQE30::&alpha;SNAP/pRep4 and got a 98,9 ng/µL DNA sample.<br/> <br/>
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Digested pQE30::&alpha;SNAP with BamHI and KpnI restriction enzymes. Separation of the gene on non interest (&alpha;SNAP) from the pQE30 vector backbone by gel electrophoresis (1.2 % agarose, 30 min, 135V).<br/> <br/>
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</p>
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                                              <h3>Thursday</h3>
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                                              <h3>Friday</h3>
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                                          <h2>Week 3</h2>
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                                          <h2>Week 4</h2>
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<p></p>
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Revision as of 22:38, 20 August 2013

Grenoble-EMSE-LSU, iGEM


Grenoble-EMSE-LSU, iGEM

Retrieved from "http://2013.igem.org/Team:Grenoble-EMSE-LSU/Documentation/Notebook/June"