Team:Grenoble-EMSE-LSU/Documentation/Notebook/May

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READ THESE INSTRUCTIONS.
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Nicolas Roehri is currently working on this page. <strong>DO NOT EDIT THIS PAGE!!!</strong> </br>
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<h1>May</h1>
<h1>May</h1>
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                                           <h2>Week 4 (20-24)</h2>
                                           <h2>Week 4 (20-24)</h2>
                                               <h3>Monday</h3><br><br>
                                               <h3>Monday</h3><br><br>
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                                               <p>We spend some time discovering the equipment at our disposal, how it works and what it can and can't do. We have access to a microplate reader (absorbance and fluorescence), a spectrophotometer (absorbance), an inverted fluorescence microscope, a PCR machine an, multiple centrifuges and electrophoresis machines, and plenty of pipetmans, plastic disposables and gloves. I'm sure there's a couple of things I've forgotten on the list, but at least we know we have what's necessary to start on great footing.<br><br>
                                               <p>We spend some time discovering the equipment at our disposal, how it works and what it can and can't do. We have access to a microplate reader (absorbance and fluorescence), a spectrophotometer (absorbance), an inverted fluorescence microscope, a PCR machine an, multiple centrifuges and electrophoresis machines, and plenty of pipetmans, plastic disposables and gloves. I'm sure there's a couple of things I've forgotten on the list, but at least we know we have what's necessary to start on great footing.<br><br>
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                                               We have transformed fresh cells with a GFP gene controlled by a PBad promoter using <b>INSERT PROTOCOL NAME HERE</b></p>
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                                               We have transformed fresh cells with a GFP gene controlled by a PBad promoter using <a href="https://static.igem.org/mediawiki/2013/f/fe/Grenoble_Protocols-TSS_Transformations.pdf"> the TSS method</a></p>
                                               <h3>Wednesday</h3><br><br>
                                               <h3>Wednesday</h3><br><br>
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                                               <h3>Wednesday</h3>
                                               <h3>Wednesday</h3>
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                                                   <br><br><p>In order to obtain better results in terms of fluorescence and in terms of bacterial growth control, we choose to use M9 for a second experiment to characterize PBAD.<br><br>
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                                                   <br><br><p>In order to obtain better results in terms of fluorescence and in terms of bacterial growth control, we choose to use <a href="https://static.igem.org/mediawiki/2013/5/50/Grenoble_Preparation_of_M9_medium.pdf">M9</a> for a second experiment to characterize PBAD.<br><br>
M9 is supplemented in glucose. We use <em>E. coli</em> BW25113 which is a prototroph.<br><br>
M9 is supplemented in glucose. We use <em>E. coli</em> BW25113 which is a prototroph.<br><br>
The protocol used is available on the internet. The experiment failed for multiple reasons including lack of controls, lack of time to do every step every 30 minutes.
The protocol used is available on the internet. The experiment failed for multiple reasons including lack of controls, lack of time to do every step every 30 minutes.

Latest revision as of 01:00, 5 October 2013

Grenoble-EMSE-LSU, iGEM


Grenoble-EMSE-LSU, iGEM

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