Team:Grenoble-EMSE-LSU/Project/Instrumentation/Fluo

From 2013.igem.org

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<p>The box has been made to be mountable in an incubator. On its support, there are holes that fit the holes of the incubator so that you can simply screw them together. There are also two doors, one with a latch that enables us to easily put the Erlenmeyer and a second dedicated to the electronic circuitry and optical components. As a safety and experimental issue, the two parts are completely separated to avoid spreading the culture on the electronic part or illuminating the culture with an unwanted wavelength. There is only one hole that allows us to illuminate the sample.</p>
<p>The box has been made to be mountable in an incubator. On its support, there are holes that fit the holes of the incubator so that you can simply screw them together. There are also two doors, one with a latch that enables us to easily put the Erlenmeyer and a second dedicated to the electronic circuitry and optical components. As a safety and experimental issue, the two parts are completely separated to avoid spreading the culture on the electronic part or illuminating the culture with an unwanted wavelength. There is only one hole that allows us to illuminate the sample.</p>
<h3>The filter rack and the rail</h3>
<h3>The filter rack and the rail</h3>
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<p><img src="https://static.igem.org/mediawiki/2013/f/fb/Talk%27E_filter.png" alt="filter_rack_inside_box" style="float:left" width="300px"/>To create the filter rack we were inspired by a cube filter which is composed by two excitation filters – green and blue – two emission filters – red and yellow – and two dichroic mirrors.  We re-design it on SketchUp by adding two more slots. The first slot is used to measure the red fluorescence of KillerRed. There is a green excitation filter on the top, a red emission filter on one side and a dichroic mirror between the two pieces.  A red colored filter is on the top of the second slot to induce the KillerRed protein production. There is no filter in the third slot because it is used to activate ROS emission with white light. Since the light comes from above there is a plate mirror between the two pieces under the slot two and three. The last slot was planned for further use, for instance to measure back-scattering of the cell suspension. For such measure, a colored filter and a half-reflecting mirror would be used. Back-scattering would provide information about the total number of bacteria, similar to OD600nm recording.</br>
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<p><img src="https://static.igem.org/mediawiki/2013/f/fb/Talk%27E_filter.png" alt="filter_rack_inside_box" style="float:left;margin: 50px;" width="300px"/>To create the filter rack we were inspired by a cube filter which is composed by two excitation filters – green and blue – two emission filters – red and yellow – and two dichroic mirrors.  We re-design it on SketchUp by adding two more slots. The first slot is used to measure the red fluorescence of KillerRed. There is a green excitation filter on the top, a red emission filter on one side and a dichroic mirror between the two pieces.  A red colored filter is on the top of the second slot to induce the KillerRed protein production. There is no filter in the third slot because it is used to activate ROS emission with white light. Since the light comes from above there is a plate mirror between the two pieces under the slot two and three. The last slot was planned for further use, for instance to measure back-scattering of the cell suspension. For such measure, a colored filter and a half-reflecting mirror would be used. Back-scattering would provide information about the total number of bacteria, similar to OD600nm recording.</br>
The filters were taken from the cube filter we receive but the plate mirror was created in a clean room by aluminum sputtering  at 70W and 1.2Pa – the thickness of the aluminum is about 20nm.
The filters were taken from the cube filter we receive but the plate mirror was created in a clean room by aluminum sputtering  at 70W and 1.2Pa – the thickness of the aluminum is about 20nm.
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</p>

Revision as of 13:17, 4 October 2013

Grenoble-EMSE-LSU, iGEM


Grenoble-EMSE-LSU, iGEM

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