Team:Grenoble-EMSE-LSU/Project/Modelling/Density

From 2013.igem.org

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<p>$1$ Cells are first incubated in the dark during a first period of approximately 100 minutes without illumination to let the bacteria grow and produce KillerRed.<p>
<p>$1$ Cells are first incubated in the dark during a first period of approximately 100 minutes without illumination to let the bacteria grow and produce KillerRed.<p>
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<p>$2$ During a second period, the cell suspension was illuminated at maximal intensity ($I=1). This makes it possible to determine precisely the capricious parameters ($b$, $k$ and $l$). The length of this period depends on the precision wanted for the parameters: the longer it lasts, the largest the effects of light are, and more efficiently is the fit improved.</p>
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<p>$2$ During a second period, the cell suspension was illuminated at maximal intensity ($I=1$). This makes it possible to determine precisely the capricious parameters ($b$, $k$ and $l$). The length of this period depends on the precision wanted for the parameters: the longer it lasts, the largest the effects of light are, and more efficiently is the fit improved.</p>
<p>$3$ Once the parameters are chosen, the model is used to determinate the light intensity predicted to stabilize our system.</p>
<p>$3$ Once the parameters are chosen, the model is used to determinate the light intensity predicted to stabilize our system.</p>

Revision as of 13:28, 3 October 2013

Grenoble-EMSE-LSU, iGEM


Grenoble-EMSE-LSU, iGEM

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