Team:Grenoble-EMSE-LSU/Project/Modelling/Density

From 2013.igem.org

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<p> And the initial contitions were:</p>
<p> And the initial contitions were:</p>
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<p> $OD_{600}=0.015%</p>
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<p> $OD_{600}=0.015$</p>
<p> $fluorescence=0UF$</p>
<p> $fluorescence=0UF$</p>
   
   
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<p>Here the predictions of the amount of living cells (in $OD_{600}$). If the light intensity is too strong, all the bacteria die, if it is too weak, they grow. And there is a value of light intensity in the middle that permits to get a constant value of living cells. </p>
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<p>In this first figure, the density of living cells $C(t)$ is displayed in $OD_{600} units. When the light intensity is too strong, all bacteria die, when it is too weak, they grow exponentially. A particular value of the light intensity allows to get a constant density of living cells. Note that this light intensity {I*} should be precisely regulated, since 10% increase or decrease around this particular light intensity will result in a X% decrease or increase of the living cell density after 10h. </p>
<center><img src="https://static.igem.org/mediawiki/2013/2/2c/Control_3_cells.png"></center>
<center><img src="https://static.igem.org/mediawiki/2013/2/2c/Control_3_cells.png"></center>
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<p id="ODprev">Here is the prediction of the global $OD_{600}$, with both living and dead bacteria. When the light intensity is too strong, the $OD_{600}$ turns constant, when light is too weak, it growths exponentially. But on the right value of light, it growths in a linear way.</p>
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<p id="ODprev">The evolution of $OD_{600} is due to both living and dead bacteria. When the light intensity is too strong, the OD600 tends to a constant, when the light intensity is too weak, it growths exponentially. At the light intensity $I*$, $OD_{600}$ increases linearly.</p>
<center><img src="https://static.igem.org/mediawiki/2013/b/ba/Control_3_OD600.png"></center>
<center><img src="https://static.igem.org/mediawiki/2013/b/ba/Control_3_OD600.png"></center>
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<p></p>
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<p>The evolution of KillerRed fluorescence is also very sensitive to the light intensity. It decreases when the illumination is too strong, increases rapidly when it is too weak, and increases more slowly at the stabilizing light intensity $I*$. </p>
<center><img src="https://static.igem.org/mediawiki/2013/a/af/Control_3_fluo.png"></center>
<center><img src="https://static.igem.org/mediawiki/2013/a/af/Control_3_fluo.png"></center>

Revision as of 12:46, 3 October 2013

Grenoble-EMSE-LSU, iGEM


Grenoble-EMSE-LSU, iGEM

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