Team:Grenoble-EMSE-LSU/Project/Validation/Future

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<h1>Future Experiments</h1>
<h1>Future Experiments</h1>
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<p>We still have three experiments to run before the completion of our project:</p>
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<p>We still have three experiments to perform in order to complete of our project:</p>
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<p>One that will be the same that, our proof of concept, to ensure it.</p>
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<p>One to show that the concentration of bacteria can be stabilized without any other variable measurable than the fluorescence.</p>
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<p>A last one to test the device, and show it can run automatically the second experiment.</p>
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<h2>Second Proof of Concept</h2>
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<p> This proof of concept is a key of the project : we have to show it is possible to control a population with our genetic network.</p>
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<h3> Voigt Plasmid and degradation tag</h3>
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<h2>Control with the fluorescence</h2>
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<p>To test the light-sensitive promoters for the expression and degradation of KillerRed and to show that the KillerRed concentration can be controlled both positively and negatively. </p>
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<p> As the device is not able to measure the absorbance of the solution, it is important to test the precision of the model predictive control when its only available measure is the fluorescence. It will be the same experiment than the proof of concept, but the correction on the light intensity shall be calculated with only the fluorescence as guide.</p>
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<h3>Control with the fluorescence alone</h3>
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<h2>Test of the Voigt Plasmid</h2>
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<p> To demonstrate that the bacterial concentration can be stabilized without any other variable measurable than KillerRed fluorescence. This is essentially the same experiment as the one described in the <a href="https://2013.igem.org/Team:Grenoble-EMSE-LSU/Project/Validation">proof of concept</a> experiment but the correction on the light intensity will be calculated with only KillerRed fluorescence as a guide.</p>
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<p></p>
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<h3>Test of the Device</h3>
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<p>To test the device, and to show that a model predictive control algorithm is able to automatically run the experiment. A Java program will be written to automatize the manual procedure that we employed for the <a href="https://2013.igem.org/Team:Grenoble-EMSE-LSU/Project/Validation">proof of concept</a> experiment .</p>
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<h2> Perspectives</h2>
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<h3> Studying bacterial resistance to oxidative stress</h3>
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<p>The possibility to independently control the amount of KillerRed expressed in the cells and its phototoxic activity will allow us to obtain cell suspension growing at a precisely defined oxidative stress. Our device indeed allows controlling the ROS production rate in living bacteria. In the frame of our model, this means making the quantity $\frac{IK}{C}$ constant. Assuming that ROS disappear at a rate proportional to the ROS concentration, the ROS concentration per living cell will thus remain constant. Our bio-electronic system can thus be useful to study the influence of ROS on cell growth, and to investigate how different genes and molecule affect bacterial resistance to oxidative stress. Practically, one would first determine the specific parameters of the bacterial suspension as explained before, then the light intensity time profile will be calculated with our model and applied to the bacterial suspension. </p>
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<h2>Test of the Device</h2>
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<p></p>
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<li id="next"><a href="/Team:Grenoble-EMSE-LSU/Project/Instrumentation">Next Page</a></li>
<li id="next"><a href="/Team:Grenoble-EMSE-LSU/Project/Instrumentation">Next Page</a></li>

Latest revision as of 03:57, 5 October 2013

Grenoble-EMSE-LSU, iGEM


Grenoble-EMSE-LSU, iGEM

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