"
Team:Groningen/Labwork/8 August 2013
From 2013.igem.org
(Difference between revisions)
| Line 30: | Line 30: | ||
<br>Transformation to <i>E. coli</i> of all the ligation reactions. | <br>Transformation to <i>E. coli</i> of all the ligation reactions. | ||
| + | <h2>Claudio</h2> | ||
| + | The biofilm grew during the last few days | ||
</div> | </div> | ||
Revision as of 12:49, 8 August 2013
Mirjam
Check of the plates of the restrictions made yesterday. One of the plates with CFP showed 1 colony. The other two plates (one IPTG) did not show any. The transformation of Pdes/CheY into the backbone failed, but the transformation of Pdes/CheY/eYFP showed a couple of colonies.Inoculated the colony of CFP and four colonies of Pdes/CheY/eYFP in LB medium with amp for mini prep.
Made a new ligation reaction between Pdes and CheY.
Made a new ligation reaction between CFP and the backbone.
Made a new ligation reaction between Pdes/CheY and the backbone.
Transformation to E. coli of all the ligation reactions.
Claudio
The biofilm grew during the last few days
iGEM 2013 Groningen
|
|
|
|
 |
|
