Team:Groningen/Labwork/8 August 2013

From 2013.igem.org

Revision as of 10:11, 8 August 2013 by Mirjam (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Mirjam

Check of the plates of the restrictions made yesterday. One of the plates with CFP showed 1 colony. The other two plates (one IPTG) did not show any. The transformation of Pdes/CheY into the backbone failed, but the transformation of Pdes/CheY/eYFP showed a couple of colonies.
Inoculated the colony of CFP and four colonies of Pdes/CheY/eYFP in LB medium with amp for mini prep.
Made a new ligation reaction between Pdes and CheY.
Made a new ligation reaction between CFP and the backbone.
Made a new ligation reaction between Pdes/CheY and the backbone.
Transformation to E. coli of all the ligation reactions.