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Team:Groningen/Labwork/9 July 2013
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(Created page with "'''Mirjam''' <br/>PCR made for silk without strep-tags. <br/>Gel run for 22 min 90V. <br/>Gel reveals a small band at 900bp. That is why a new 1.5% agarosegel is run for 30 min ...") |
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<br/>PCR made for silk without strep-tags. | <br/>PCR made for silk without strep-tags. | ||
<br/>Gel run for 22 min 90V. | <br/>Gel run for 22 min 90V. | ||
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<br/>Cut wrong bands from gel. So a new PCR is made. | <br/>Cut wrong bands from gel. So a new PCR is made. | ||
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spotted transformed cells and put them in a 37 C shaker (at 11:35) | spotted transformed cells and put them in a 37 C shaker (at 11:35) | ||
Revision as of 11:59, 24 July 2013
Mirjam
PCR made for silk without strep-tags.
Gel run for 22 min 90V.
Gel reveals a small band at 900bp. That is why a new 1.5% agarosegel is run for 30 min at 90V.
Cut wrong bands from gel. So a new PCR is made.
Inne
spotted transformed cells and put them in a 37 C shaker (at 11:35) Cells need to grow over night.
15:00 PCR in order to get more silk gene to cut out of gel(last one was cut to low)
