Team:Groningen/Labwork/9 July 2013

From 2013.igem.org

(Difference between revisions)
Line 1: Line 1:
 +
<html>
 +
<head>
 +
<meta http-equiv="X-UA-Compatible" content="IE=EDGE" charset="utf-8" />
 +
<link rel="stylesheet" href="https://2013.igem.org/Team:Groningen/CSS/DefaultPage?action=raw&ctype=text/css" type="text/css" />
 +
</head>
 +
<body>
 +
 +
 +
<div id="header">
 +
  <img src="https://static.igem.org/mediawiki/2013/8/83/Groningen_slider2.jpg" width=100% height="220" >
 +
</div>
 +
 +
<div class="colmask threecol">
 +
 +
<span class="navigation">
 +
    </html>{{:Team:Groningen/Templates/Navigationbar}}<html>
 +
</span>
 +
 +
<span class="widgets">
 +
    </html>{{:Team:Groningen/Templates/facebook}}<html>
 +
</span>
 +
 +
<div class="mainContent">
 +
   
 +
<h2>Mirjam</h2>
<h2>Mirjam</h2>
<br/>PCR made for silk without strep-tags.  
<br/>PCR made for silk without strep-tags.  
Line 11: Line 36:
15:00 PCR in order to get more silk gene to cut out of gel(last one was cut to low)
15:00 PCR in order to get more silk gene to cut out of gel(last one was cut to low)
 +
   
 +
   
 +
</div>
 +
 +
 +
</div>
 +
 +
<div id="footer">
 +
  <p>iGEM 2013 Groningen</p>
 +
</div>
 +
 +
</body>
 +
</html>

Revision as of 11:59, 24 July 2013

Mirjam


PCR made for silk without strep-tags.
Gel run for 22 min 90V.
Gel reveals a small band at 900bp. That is why a new 1.5% agarosegel is run for 30 min at 90V.
Cut wrong bands from gel. So a new PCR is made.

Inne

spotted transformed cells and put them in a 37 C shaker (at 11:35) Cells need to grow over night. 15:00 PCR in order to get more silk gene to cut out of gel(last one was cut to low)

Retrieved from "http://2013.igem.org/Team:Groningen/Labwork/9_July_2013"