Team:Groningen/Labwork/9 July 2013

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(Created page with "'''Mirjam''' <br/>PCR made for silk without strep-tags. <br/>Gel run for 22 min 90V. <br/>Gel reveals a small band at 900bp. That is why a new 1.5% agarosegel is run for 30 min ...")
 
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'''Mirjam'''
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<br/>PCR made for silk without strep-tags.  
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<br/>Gel run for 22 min 90V.
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<h2>Mirjam</h2>
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<br/><a href="https://2013.igem.org/Team:Groningen/protocols/PCR"><FONT COLOR="black"><b>PCR</b></FONT></a> made for silk without strep-tags.  
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<br/><a href="https://2013.igem.org/Team:Groningen/protocols/GelElectrophoresis"><FONT COLOR="black"><b>gel</b></FONT></a> run for 22 min 90V.
<br/>Gel reveals a small band at 900bp. That is why a new 1.5% agarosegel is run for 30 min at 90V.
<br/>Gel reveals a small band at 900bp. That is why a new 1.5% agarosegel is run for 30 min at 90V.
<br/>Cut wrong bands from gel. So a new PCR is made.
<br/>Cut wrong bands from gel. So a new PCR is made.
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''' Inne'''
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<h2>Inne</h2>
spotted transformed cells and put them in a 37 C shaker (at 11:35)
spotted transformed cells and put them in a 37 C shaker (at 11:35)
Cells need to grow over night.
Cells need to grow over night.
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15:00 PCR in order to get more silk gene to cut out of gel(last one was cut to low)
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15:00 Did a <a href="https://2013.igem.org/Team:Groningen/protocols/PCR"><FONT COLOR="black"><b>PCR</b></FONT></a> in order to get more silk gene to cut out of gel(last one was cut to low)
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Latest revision as of 10:02, 30 July 2013

Mirjam


PCR made for silk without strep-tags.
gel run for 22 min 90V.
Gel reveals a small band at 900bp. That is why a new 1.5% agarosegel is run for 30 min at 90V.
Cut wrong bands from gel. So a new PCR is made.

Inne

spotted transformed cells and put them in a 37 C shaker (at 11:35) Cells need to grow over night. 15:00 Did a PCR in order to get more silk gene to cut out of gel(last one was cut to low)

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