Team:Groningen/protocols/Digestion
From 2013.igem.org
(Difference between revisions)
Line 36: | Line 36: | ||
<h3>Reaction:</h3> | <h3>Reaction:</h3> | ||
- | + | <table border="1"> | |
- | + | <tr> | |
- | + | <th>Compound</th> | |
- | + | <th>20µl</th> | |
- | + | <th>Final concentration</th> | |
- | + | </tr> | |
- | + | ||
- | < | + | <tr> |
- | + | <td>MQ water</td> | |
- | <td> | + | <td>up to 20µl</td> |
- | <td> | + | <td></td> |
- | </tr> | + | </tr> |
- | <tr><td> | + | |
- | <td> | + | <tr> |
- | <td></td> | + | <td>10x buffer</td> |
- | </tr> | + | <td>2µl</td> |
- | <tr><td> | + | <td>1x</td> |
- | <td> | + | </tr> |
- | <td> | + | |
- | </tr> | + | <tr> |
- | <tr><td>DNA</td> | + | <td>restriction enzyme</td> |
- | <td>4& | + | <td>1µl</td> |
- | <td>up to 1& | + | <td>1U</td> |
- | </tr> | + | </tr> |
- | + | ||
- | + | <tr> | |
- | + | <td>DNA</td> | |
- | + | <td>4µl</td> | |
- | </table> | + | <td>up to 1µl</td> |
+ | </tr> | ||
+ | |||
+ | </table> | ||
<br>All the reagents are added following the order listed in the table above. | <br>All the reagents are added following the order listed in the table above. |
Revision as of 13:57, 27 July 2013
Digestion
Fast digest
Materials:
- MQ water
- 1.5ml tubes
- Fast digestion buffer
- Restriction enzymes
- DNA
Reaction:
Compound | 20µl | Final concentration |
---|---|---|
MQ water | up to 20µl | |
10x buffer | 2µl | 1x |
restriction enzyme | 1µl | 1U |
DNA | 4µl | up to 1µl |
All the reagents are added following the order listed in the table above.
After the reaction is ready mix the content of the tube and spin it down.
The tubes are incubated for 1h at 37°C.
Reference: http://www.thermoscientificbio.com/uploadedFiles/Resources/fast-digestion-dna.pdf