Team:Groningen/protocols/Digestion

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Digestion

Materials:

H₂O
1.5ml tubes
Ligation buffer
Restriction enzymes
DNA

Reaction:

Component	30μl reaction	Final concentration
H₂O	up to 30μl
H₂O	up to 30μl
Buffer 10x	3	1x
DNA	4μl	up to 1μg
Enzyme/s	0.5