Digestion

Fast digest

Materials:

• MQ water
• 1.5ml tubes
• Fast digestion buffer
• Restriction enzymes
• DNA

Reaction:

Compound	20µl	Final concentration
MQ water	up to 20µl
10x buffer	2µl	1x
restriction enzyme	1µl	1U
DNA	4µl	up to 1µl

All the reagents are added following the order listed in the table above.
After the reaction is ready mix the content of the tube and spin it down.
The tubes are incubated for 1h at 37°C.

Reference: http://www.thermoscientificbio.com/uploadedFiles/Resources/fast-digestion-dna.pdf