Team:HIT-Harbin

From 2013.igem.org

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<p>The following picture is the whole design of our circuit.</p>
<p>The following picture is the whole design of our circuit.</p>
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<img src="http://2013.igem.org/wiki/images/7/75/HIT-Harbin_Project_Andgate.png" />
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<img src="http://2013.igem.org/wiki/images/7/75/HIT-Harbin_Project_Andgate.png" width="500" />
<p>Figure 1 : A Schematic of the Biological Andgate</p>
<p>Figure 1 : A Schematic of the Biological Andgate</p>
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<p>As showed in Fig. 1, a protein complex HrpRS is formed following the combination of HrpS and HrpR. HrpRS then binds to the promoter hrpL, triggering transcription of the downstream gene. Two inducible promoters--the isopropylthiogalactoside (IPTG)-inducible Plac and the arabinose-inducible PBAD – are selected as the AND gate inputs. A model based on protein and protein-promoter interactions for the hrp gene regulation is described below:</p>
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<p>As showed in Fig. 1, a protein complex HrpRS is formed following the combination of HrpS and HrpR. HrpRS then binds to the promoter hrpL, triggering transcription of the downstream gene. Two inducible promoters--the isopropylthiogalactoside (IPTG)-inducible Plac and the arabinose-inducible PBAD...</p>

Revision as of 19:10, 24 September 2013

HIT-Harbin

HIT-Harbin

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The circuit

The following picture is the whole design of our circuit.

Figure 1 : A Schematic of the Biological Andgate

As showed in Fig. 1, a protein complex HrpRS is formed following the combination of HrpS and HrpR. HrpRS then binds to the promoter hrpL, triggering transcription of the downstream gene. Two inducible promoters--the isopropylthiogalactoside (IPTG)-inducible Plac and the arabinose-inducible PBAD...

Learn More

Click here and get more details about our project.